人乳β-酪蛋白酶解产物体外对小鼠淋巴细胞的刺激作用  

In vitro human β-casein hydrolysate stimulate spleen lymphocyte of mouse proliferation and secretion of cytokine

作　　者：顾鑫[1,2] 任皓威[1] 李萌[1] 刘宁[1,2] 

机构地区：[1]国家乳业工程技术研究中心、黑龙江省乳品工业技术开发中心,哈尔滨150028 [2]东北农业大学食品学院,哈尔滨150030

出　　处：《中国乳品工业》2014年第4期8-10,41,共4页China Dairy Industry

基　　金：“十二五”国家科技支撑计划(2011BAD09B03);食品安全与营养协同创新中心项目

摘　　要：采用离子交换色谱法从人乳中分离、纯化得到人乳β-酪蛋白,利用胰蛋白酶对其进行酶解,四甲基偶氮唑盐(MTT)法检测不同孵育时间下,不同质量浓度的酶解产物对刀豆蛋白A(Con A)诱导的小鼠脾T淋巴细胞增殖情况的影响;ELISA法测定Th1型细胞因子(IL-2、IFN-γ)、Th2型细胞因子(IL-4、IL-6)分泌量。结果表明,人乳β-酪蛋白酶解产物对ConA诱导的小鼠脾淋巴细胞增殖具有显著的促进作用,且48 h效果最佳;对Th1型细胞因子(IL-2、IFN-γ)的分泌具有显著的促进作用、对Th2型细胞因子(IL-6)分泌有显著下调作用;显著增加IFN-γ/IL-4的比值。Human β-casein was obtained by anion exchange chromatography and the hydrolysate was derived from the enzymatic trypsin- hydrolysate of human β-casein. The effects of treatments with humanβ -casein hydrolysate containing Con-A at different on spleen lymphocyte proliferation under different culture times were determined by MTT method. The contents of IL-2, IFN-γ IL-4 and IL-6 were determined by ELISA. The results showed that human B-casein hydrolysate promoted Con-A-induced lymphoproliferation, and 48 h administration gave the best proliferation. It could markedly increase the type 1 cytokine （IL-2, IFN-γ） production, and could markedly down-regulate the type 2 cytokine （IL-6）, it could improve the IFN-γ/IL-4 value markedly.

关 键 词：人乳β--酪蛋白酶解产物 淋巴细胞增殖 细胞因子 TH1 Th2细胞平衡 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]