玉米耐深播相关性状的全基因组关联分析  

Genome-Wide Association Analysis of Deep-Planting Related Traits in Maize

作　　者：陈芳[1,2] 张进艳 李亮[2] 马艺文[2] 王振萍[2] 段运平 郝转芳[2] 

机构地区：[1]山西大学生物工程学院,山西太原030000 [2]中国农业科学院作物科学研究所,北京100081 [3]山西农业科学院作物科学研究所,山西太原030000

出　　处：《作物杂志》2014年第2期43-47,共5页Crops

基　　金：国家自然科学基金(31271735);国家“863”计划(2011AA 100501)

摘　　要：在干旱、半干旱地区,培育耐深播玉米品种对于玉米生产有十分重要的意义。对165份玉米自交系中胚轴长等耐深播相关性状进行调查,结合平均分布于玉米基因组的41 101个单核苷酸多态性(SNP)标记进行全基因组关联分析,筛选出5个与性状显著关联的SNP(P<0.0001),分别位于染色体bin10.03和bin5.04。对候选基因分析发现,其中4个SNP编码产物分别为酰基载体蛋白、含plus-3结构域蛋白、含FAD结构域蛋白和过氧化物酶亚家族蛋白。研究将为耐深播性状相关基因的克隆奠定基础。Breeding maize varieties of deep-planting has very important practical significance in arid and semiarid regions. In this study, the related traits in a panel of 165 maize inbred lines were detected at germination stage, and used to perform the genome-wide analysis using 41 101 SNP of MaizeSNP50 BeadChip. A total of five SNPs were found to be significantly associated with the related traits at a threshold of P 〈 0. 0001, among which three SNPs were on chromosome bin 10.03 ,two on chromosome bin 5.04. Meanwhile, four candidate genes were scanned：encoding acyl carrier prot peroxidase superfamil ein （ACP4）, plus-3 domain-containing protein, FAD binding domain-containing protein and y protein, respectively. These identified genes and SNPs will lay a foundation for cloning deep- planting genes in maize.

关 键 词：玉米 耐深播 中胚轴 全基因组关联分析 候选基因 

分 类 号：S513[农业科学—作物学]