广东部分岛屿紫菜物种研究  

Study of Porphyra species from some islands of Guangdong Province

作　　者：邓银银[1,2] 陆勤勤[3] 陈伟洲[4] 张涛[2] 田翠翠[1,2] 朱建一[2] 

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123 [2]常熟理工学院生物与食品工程学院,江苏常熟215500 [3]江苏省海洋水产研究所,江苏南通226007 [4]汕头大学海洋生物研究所,广东汕头515063

出　　处：《南方水产科学》2014年第2期11-17,共7页South China Fisheries Science

基　　金：国家自然科学基金项目(31272664);国家高技术研究发展计划(863计划)项目(2012AA10A406-6);海洋公益性行业专项(201105023)

摘　　要：为了解广东沿海紫菜物种自然资源现状及物种间的亲缘关系,于2012年冬季对广东3个岛屿进行调查,并对采集样品进行形态、细胞学以及基于rbcL和nrSSU序列的系统学研究。结果显示,12月平屿岛优势种有坛紫菜(Porphyra haitanensis)、长紫菜(P.dentata)和皱紫菜(P.crispata);外伶仃岛、下川岛为皱紫菜和多枝紫菜(P.ramosissima);3个岛屿的紫菜属于暖温性的刺缘组紫菜,染色体均为n=5,其中多枝紫菜染色体为首次观察报道。系统学结果显示,平屿岛皱紫菜与坛紫菜亲缘关系最近,与多枝紫菜之间的关系最远;外伶仃岛、下川岛皱紫菜与平屿岛皱紫菜的相似度远低于与多枝紫菜种间的系数。研究扩增了皱紫菜的nrSSU序列和多枝紫菜的rbcL、nrSSU序列,为紫菜的系统学研究提供了基础资料。To understand the natural distribution and genetic relationship of Porphyra species in coastal areas of Guangdong Province, we collected samples from Pingyu Island, Wailingding Island and Xiachuan Island of Guangdong Province in December, 2012 for DNA sequence analysis of the large subunit of ribulose-1-5-bisphoshoate carboxylase/oxygenase enzyme （rbcL） and nuclear SSU ribosomal RNA （nrSSU） gene as well as cytological observations. The results show that P. haitanensis, P. dentata and P. crispata were the domi- nate species from Pingyu Island, while P. crispata and P. ramosissima were the dominate species from Wailingding and Xiachuan Islands. All the four species, which were all warm-temperature species and belonged to Porphyra, had five chromosomes （n = 5） with undulated margin. The chromosome of P. ramosissima was firstly reported within Porphyra spp. Based on the analysis of DNA se- quences of rbcL and nrSSU, P. crispata from Wailingding and Xiachuan Islands had much closer relationship with P. crispata from Pingyu Island, while P. crispata from Pingyu Island had the closest genetic relationship with P. haitanensis and the farthest relationship with P. ramosissima. The rbcL and nrSSU genes of P. ramosissima and the nrSSU genes of P. crispata had been firstly amplified in this study, which provides basic data for the systematics of Porphyra.

关 键 词：紫菜 染色体 RBCL nrSSU 

分 类 号：S932.7[农业科学—渔业资源]