微囊藻和小球藻携带WSSV量的变化及对水体游离WSSV的影响  

Dynamic changes of WSSV carried by Microcystis aeruginosa and Chlorella pyrenoidosa and their effects on WSSV in water

作　　者：李凡[1,2] 曹煜成[2] 胡晓娟[2] 王善龙[1,2] 文国樑[2] 吴垠[1] 李卓佳[2] 

机构地区：[1]大连海洋大学,辽宁大连116023 [2]中国水产科学研究院南海水产研究所、农业部南海渔业资源开发利用重点实验室、广东省渔业生态环境重点实验室,广东广州510300

出　　处：《南方水产科学》2014年第2期54-60,共7页South China Fisheries Science

基　　金：现代农业产业技术体系建设专项资金(CARS-47);公益性行业(农业)科研专项(201103034);广东省科技计划项目(2012B020308003);中央级科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2013ZD02);农业科技成果转化资金项目(2012GB23260551);广西科学研究与技术开发计划(桂科攻13470002-2)

摘　　要：研究了铜绿微囊藻(Microcystis aeruginosa)和蛋白核小球藻(Chlorella pyrenoidosa)携带白斑综合症病毒(white spot syndrome virus,WSSV)数量变化及对水体游离WSSV量的影响。试验设置微藻高、低密度组和不加微藻的对照组,分别加入等量的WSSV粗提液,于第2、第24、第72和第120小时以实时荧光定量PCR检测藻液上清和沉淀藻体的WSSV数量。结果表明,微囊藻和小球藻可携带少量WSSV,且随时间延长而减少;微囊藻携带WSSV量与其细胞数呈显著正相关(P<0.05),小球藻携带WSSV量与其细胞数相关性不显著(P>0.05);2种微藻均有促进水体WSSV数量消减的效果,且小球藻的消减效果优于微囊藻,对养殖对虾白斑综合症(white spot syndrome,WSS)的生态防控更有积极意义。To investigate the dynamic changes of WSSV （white spot syndrome virus） carried by Microcystis aeruginosa and Chlorella pyrenoidosa and their effects on WSSV amount in water, we added the same WSSV amount to high-density groups, low-density groups with those two microalgae and compared them with the control group without microalgae, then determined the WSSV amount in super- natant and precipitate algae by TaqMan real-time PCR at 2nd hour, 24~h hour, 72＇h hour and 120th hour. Results show that both M. aeruginosa and C. pyrenoidosa carried WSSV but the amount decreased with time. The WSSV amount carried by M. aeruginosa was significantly positively correlated with the density of algae cells （ P 〈 0. 05 ） , while there was no significant correlation between the WSSV amount carried by C. pyrenoidosa and the numbers of algae cells （ P 〉 0. 05 ）. Both the two microalgae reduced WSSV amount in water, and the reduction effect of C. pyrenoidosa was better than that of M. aeruginosa, which is beneficial to the ecological preventionand control of WSS （white spot syndrome）.

关 键 词：微囊藻 小球藻 WSSV携带量 TAQMAN real—time PCR 

分 类 号：Q178[生物学—水生生物学] S945.1[生物学—普通生物学]