七氟醚预处理对大鼠急性肾缺血-再灌注损伤保护作用的实验研究  

Sevoflurane preconditioning on the experimental study on the protective effects of acute renal ischemia-reperfusion injury in rats

作　　者：田德明[1] 叶虹[1] 冯微[1] 

机构地区：[1]吉林大学中日联谊医院麻醉科,吉林长春130033

出　　处：《中国实验诊断学》2014年第4期530-533,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探索七氟醚对急性肾缺血-再灌注损伤肾功能的保护作用。方法选择健康SD大鼠90只,随机分为三组:伪手术组、对照组及七氟醚预处理组,建立肾缺血-再灌注模型,测定各组大鼠平均动脉压(MAP)、二氧化碳分压(PCO2)、血清尿素氮(BUN)、肌酐(Cr)及超氧化物歧化酶(SOD)的变化,HE染色观察各组大鼠肾组织病理改变。结果随着七氟醚预处理浓度的增加,大鼠MAP呈下降趋势,但在七氟醚浓度2%-3%的临床常用剂量范围内,大鼠的MAP波动于92.1±6.0mmHg。各组肾缺血前与再灌注后,PCO2浓度无明显变化。对照组及七氟醚预处理组BUN和Cr于再灌注后12h、24h均明显高于伪手术组(P<0.05),但七氟醚预处理组再灌注后12h、24h的BUN、Cr水平均较对照组明显下降(P<0.05)。七氟醚预处理组和对照组均较伪手术组血清SOD活力减低,但七氟醚预处理组SOD活力较对照组高,差异具有统计学意义(P<0.05)。肾脏病理观察发现伪手术组肾脏组织结构完整,对照组肾脏外髓部分组织结构严重破坏,而七氟醚预处理组肾脏组织破坏较小,肾小管组织相对完整。结论七氟醚吸入式麻醉能够有效降低BUN、Cr水平,保护SOD活力,对急性肾缺血-再灌注损伤肾功能具有一定的保护作用。Objective To explore the protective effects of sevoflurane on renal function in acute renal ischemia-reperfusion injury.Methods 90 healthy SD rats were randomly divided into three groups：sham-operated group,control group,sevoflurane inhalated group.Acute renal ischemia reperfusion model were established by clamping the renal pedi-cle.Mean arterial pressure（MAP）,partial pressure of carbon dioxide（PCO2 ）,blood urea nitrogen（BUN）,creatinine （Cr）and superoxide dismutas-e（SOD）levels were determined in each groups,the renal pathological changes were ob-served by HE staining.Results With the increase in the concentration ofsevoflurance,the mean arterial pressure of the rats decreased,But the usual clinical doses in the range of sevoflurane concentration in 2%-3%,MAP is 92.1 ＆#177; 6.0 mmHg.There is no significant difference of PCO2 concentration in each group before ischemia and after reperfusion.Af-ter renal ischemia reperfusion 12 h,24 h,the BUN and Cr levels of control group and sevoflurane inhalated group were significantly higher than that in sham-operated group （P 〈0.05）,but the BUN,Cr levels of sevoflurane inhalated group were decreased significantly compared with B group （P 〈0.05）.Compared with sham-operated group,the serum SOD activity of control group and sevoflurane inhalated group were significantly decreased （P 〈0.05）,But the activity of SOD of sevoflurane inhalated group was higher than control group （P 〈 0.05）.The renal pathological structure of sham-operated group was integrity,the damage of renal outer medullary tissue was severly in control group,but the sevoflurane inhalated group kidney tubular tissue damage were lightly.Conclusion Sevoflurane inhalation anesthesia can effectively reduce BUN,Cr level,protect SOD activity,and thus,has a protective effect on renal function on acute renal ischemia/reperfusion injury.

关 键 词：七氟醚 缺血-再灌注损伤 肾功能 

分 类 号：R363[医药卫生—病理学]