基于普鲁士蓝/聚多巴胺/纳米金纳米粒子构建的电流型免疫传感器  

A Label-Free Electrochemical Immunosensor Based on Gold Nanoparticles-Polydopamine-Prussian Blue Nanocomposite for Determination of α-Fetoprotein

作　　者：潘伯广 洪成林[1] 陈晓玉[1] 赵云[1] 

机构地区：[1]石河子大学化学化工学院,新疆生产建设兵团化工绿色过程重点实验室,省部共建国家重点实验室培育基地,新疆石河子832000 [2]石河子大学药学院,新疆石河子832000

出　　处：《广东化工》2014年第8期119-120,126,共3页Guangdong Chemical Industry

基　　金：农药拟除虫菊酯残留的电化学生物传感器研究及应用(21065009)

摘　　要：首先制备普鲁士蓝纳米粒子（PB）,通过利用多巴胺（DA）自氧化聚合形成聚多巴胺膜（Dopa）的特性,在PB纳米粒子表面包覆成膜,有效增加PB的稳定性,并在氯金酸溶液中原位还原沉积金纳米粒子,利用纳米金固定化甲胎蛋白抗体（anti-AFP）,制得灵敏度高和稳定性好的无标记免疫传感器.采用透射电子显微镜（TEM）对纳米粒子进行表征,采用循环伏安法（CV）和交流阻抗法（EIS）考察修饰电极的电化学特性,实验结果表明,PB-Dopa-Au纳米粒子修饰的电极在实验过程中呈现出良好的氧化还原活性,对甲胎蛋白的检测范围为0.02～80 ng/mL检出限为0.01ng/mL.In the paper, a simple and highly sensitive amperometric immunosensor for the determination of alpha-fetoprotein （AFP） based on gold nanoparticles-polydopamine-Prussian Blue （PB-Dopa-Au） nanocomposites was fabricated.Prussian blue （PB） nanoparticle was initially synthesized under ultrasonic condition and it was surface functionalized by one-step oxidative polymerization of dopamine in basic solution at environment friendly condition to obtain the polydopamine （Pdop） modified reduced PB nanoparticles. Then, via in situ deposition, the Au NPs deposited on the polydopamine functionalized PB nanocomposites Finally, antibodies were anchored on the Au NPs.The preparation of the multifunctional nanoparticle （PB-Dopa-Au）） was characterized by TEM and the assembly of the biosensor was characterized with cyclic voltammetry （CV） and electrochemical impedance spectroscopy （EIS）.The dynamic range of the resulted immunosensor for the detection of AFP is from 0.02 ng/mL to 80.0 ng/mL with a detection limit of 0.02 ng/mL （S/N = 3）. Moreover, this biosensor displays good selectivity, stability and reproducibility.

关 键 词：普鲁士蓝 纳米金 甲胎蛋白 免疫传感器 

分 类 号：R741.04[医药卫生—神经病学与精神病学]