淡水绿藻在培养基中生长及油脂积累研究  

Growth and oil accumulation of microalgae strains isolated from freshwater cultured in mediums

作　　者：张森[1] 刘平怀[1] 杨勋[1] 郝宗娣[1] 张玲[1] 罗宁[1] 吴楠[1] 

机构地区：[1]海南大学材料与化工学院,热带作物种质资源保护与开发利用教育部重点实验室,海口570228

出　　处：《中国油脂》2014年第4期48-53,共6页China Oils and Fats

基　　金：国家科技支撑计划(2011BAD14B01);海南省中药现代化科技专项(ZY201327);海南大学植物学国家级重点学科资助(071001)

摘　　要：为筛选出最适合进行油脂生产的藻株及其较适生长和油脂积累的培养基,采用柱状光生物反应器进行一次性培养,对微藻Tetranephrisbrasiliensis DL12、Ankistrodesmusgracilis CJ09、Ankistrodesmus sp.CJ02及Desmodesmussubspicatus WC01在4种常用淡水绿藻培养基BG11、3N-BBM、SE及TAP中的生长和油脂积累进行研究。结果表明:3N-BBM有利于微藻的生长和生物量的积累,BG11有利于微藻细胞内油脂的积累;16组培养试验中,Tetranephrisbrasiliensis DL12在3N-BBM中的生物量产率((257.65±45.52)mg/(L·d))和油脂产率((80.34±14.19)mg/(L·d))最高,Ankistrodesmusgracilis CJ09在BG11中的油脂含量(42.72%±0.60%)最高。Tetranephrisbrasiliensis DL12最具油脂生产潜力,其最适生长和油脂积累的培养基为3N-BBM。The batch culture was conducted by column photobioreactor so as to select the microalgae most suitable for oil production and the culture medium suitable for growth and oil accumulation of the microal- gae. The growth and oil accumulation of four microalgaes（ Tetranephrisbrasiliensis DL12, Ankistrodesmus- gracilis CJ09, Ankistrodesmus sp. CJ02 and Desmodesmussubspicatus WC01 ） in four culture mediums （ BG11,3N - BBM, SE and TAP） were investigated. The results showed that the 3N - BBM medium was benefit for the growth and biomass accumulation of microalgaes; the BG11 medium was beneficial to oil accumulation of microalgaes; in sixteen groups of experiment, Tetranephrisbrasiliensis DL12 had the high- est biomass production rate（ （257.65 ±45.52）mg/（ L d）） and oil production rate（ （80.34 ± 14.19） mg/（ L d）） in 3N - BBM medium, and Ankistrodesmusgracilis CJ09 had the highest oil content （42.72% ±0.60% ） in BGll medium. Tetranephrisbrasiliensis DL12 had the largest oil production po- tential and its optimal medium for growth and oil accumulation was 3N- BBM.

关 键 词：培养基 微藻 生长 油脂积累 

分 类 号：TS222[轻工技术与工程—粮食、油脂及植物蛋白工程] S968.4[轻工技术与工程—食品科学与工程]