垂体瘤发病相关基因的大规模筛选及鉴定  

Large-Scale Screening for Genes Related to Pathogenesis of Pituitary Carcinoma

作　　者：宋怀东[1] 胡仁明[1] 黄秋花[2] 彭永德[1,3] 任双喜 周隽[2] 吴昕彦[2] 付刚 张庆华[2] 陈家伦[1] 

机构地区：[1]第二医科大学附属瑞金医院,上海市内分泌研究所,上海200025 [2]上海血液学研究所,上海200025 [3]国家人类基因组南方研究中心,上海201203

出　　处：《癌症》2001年第3期229-234,共6页Chinese Journal of Cancer

基　　金：国家自然科学基金资助项目 (30000082;39870311;39970345);卫生部B类基金资助项目 (98-2-285)

摘　　要：目的：探讨垂体瘤与垂体组织差异表达基因的大规模筛选及鉴定。方法：垂体无功能腺瘤及正常垂体组织 cDNA文库构建，大规模表达序列标签 (expressed sequence tags， ESTs)测序、表达谱差异的生物信息学处理及半定量逆转录－聚合酶链反应 (reverse transcriptase polymerase chain reaction， RT-PCR)鉴定。结果：从无功能腺瘤和正常垂体组织中分别获得 1253和 7222个质量满意的 ESTs；垂体瘤中已知基因的品种数 200个，经组织表达谱差异比较的统计学软件处理，在这些已知基因中，差异的可靠性≥ 0.99者 38个，≥ 0.95者 130个；其中与细胞生长、分化相关的 G2类基因共 17个； 6个经半定量 RT-PCR证实 4个基因的表达在垂体瘤中明显高于正常垂体。结论：与细胞生长、分化相关的 G2类基因 MEIS2、 SMT3C、 C1D、 BUB3在垂体瘤中表达高于正常垂体，推测可能与垂体瘤的发生有关。Objective： The aim of this study was to investigate the method of large-scale screening for genes differentially expressed in nonfunctional pituitary tumor and normal pituitary. Methods： cDNA libraries of nonfunctional pituitary tumor (NFPT) and human normal pituitary were constructed. Large-scale sequencing, bioinformatics and semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) were used. Significant difference of known genes expression in 2 tissues were analyzed by statistical software offered by Audic S and Claverie JM (http： //igs-server.cnrs-mrs.fr/). Results： A total of 1253 and 7222 ESTs with good quality were obtained from the tissues of NFPT and normal pituitary, respectively. Two hundred known genes were found in NFPT. Among them, 38 genes creditability of significant difference expression in the 2 tissues were over 0.99, and 130 were over 0.95. Out of the 130 differentially expressed genes in the 2 tissues, 17 genes were associated with cell differentiation and development (G2). 6 differentially expressed G2 category genes were selected and the liability of the expression significant difference in the 2 tissues was confirmed by semi-quantitative RT-PCR. Out of them, 4 were confirmed differentially expressed in the 2 tissues. Conclusion： MEIS2, SMT3C, C1D, and BUB3 genes associated with cell differentiation and development (G2) were expressed more in nonfunctional pituitary tumor than in normal pituitary. They may play a role in the pathogenesis of nonfunctional pituitary carcinoma.

关 键 词：垂体瘤 基因表达 谱 生物信息学 半定量RT-PCR 

分 类 号：R736.4[医药卫生—肿瘤]