整合素β_1对大肠癌细胞与血管内皮细胞间细胞通讯的影响  

Effects of β_1-integrins on gap junctional intercellular communication between human colorectal carcinoma cells and endothelial cells in vitro

作　　者：李祖国[1] 张进华[1] 邓永健[1] 丁彦青[1] 

机构地区：[1]第一军医大学病理教研室,广东广州510515

出　　处：《第一军医大学学报》2001年第3期171-172,F003,F004,共4页Journal of First Military Medical University

基　　金：广东省自然科学基金!(990385)

摘　　要：目的观察整合素β１在大肠癌细胞系ＬｏＶｏ细胞中的表达及其对大肠癌细胞与血管内皮间通讯的影响。方法 （１）应用免疫组化方法研究整合素β１在人大肠癌细胞中的表达；（２）应用激光漂白后荧光恢复技术研究整合素β１对大肠 癌细胞与血管内皮细胞间的缝隙连接细胞间通讯（Ｇａｐ ｊｕｎｃｔｉｏｎａｌ ｉｎｔｅｒｃｅｌｌｕｌａｒ ｃｏｍｍｕｎｉｃａｔｉｏｎ，ＧＪＩＣ）的影响。结果整合 素β１在ＬｏＶｏ细胞膜上有明确的阳性表达，部分较幼稚的ＬｏＶｏ细胞胞浆内也有阳性表达。被整合素β１单抗封闭后的 ＬｏＶｏ细胞与血管内皮细胞间的ＧＪＩＣ发生明显改变，激光漂白后平均荧光恢复率由（３９．７６±１６．９４）％降至（２０．２７±９．２１）％ （Ｐ＜０．０５）。结论整合素β１能影响大肠癌细胞与血管内皮细胞间的ＧＪＩＣ，并可能影响大肠癌的转移。Objective To observe the expression of β1-integrins in human colorectal carcinoma and study their effects on gap junctional intercellular communication (GJIC) between human colorectal carcinoma LoVo cells and endothelial cells. Meth- ods The expression of β1-integrins was measured immunohistochemically and changes in GJIC in response to β1-integrins treatment were assessed by fluorescence recovery after photobleaching (FRAP) technique. Results β1-integrins expression was observed mainly on the membrane of the LoVo cells, and the cytoplasm of a proportion of immature cells was also β1- integrins-positive. GJIC between the LoVo cells blocked by β1-integrins and the endothelial cells presented obvious changes, and the average fluorescence recovery rate after photobleaching fell from (39.76±16.94)% to (20.27±9.21)%. Conclusion β1- integrins affects GJIC between LoVo cells and foe endothelial cells, and may play an important role in the metastasis of colorectal carcmoma.

关 键 词：整合素Β1 缝隙连接细胞间通讯 肿瘤转移 细胞通信 大肠癌 

分 类 号：R735.34[医药卫生—肿瘤]