普罗布考对体外培养的主动脉平滑肌细胞NF-κB活性的影响  

Effects of probucol on the NF-κB-like activity of aortic smooth muscle cell in vitro

作　　者：顾江涛[1] 吴宗贵[1] 沈茜[2] 徐玉莲[2] 

机构地区：[1]第二军医大学长征医院心血管内科,上海200003 [2]长海医院实验诊断科

出　　处：《第二军医大学学报》2001年第2期151-154,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金资助项目 !(3 9670 3 16)

摘　　要：目的 :观察普罗布考 (probucol)对血管平滑肌细胞 (VSMCs)核因子κB(NF-κB)活性的调控作用 ,以探讨 probucol在动脉粥样硬化 (AS)和经皮腔内冠状动脉成形术后再狭窄 (RS)防治中的某些可能的作用机制。 方法 :采用电泳迁移位移测定法观察 probucol对 VSMCs NF-κB活性的影响。结果 :H2 O2 或胎牛血清 (NCS)处理 VSMCs 72 h后 ,NF-κB活性均明显增强 ;而加入 10 0 μm ol/ L probucol后 ,NF- κB活性受到部分抑制 ,抑制率分别为 37.1%和 14.8%。 结论 :Probucol抑制 NCS、H2 O2 诱导的 VSMCs NF- κB的活性 ,可能是其临床有效防治 RS及Objective:To observe the inhibitory effects of probucol on nuclear factor κB (NF κB) like activity of vascular smooth muscle cells(VSMCs). Methods:The effects of probucol and H 2O 2 on NF κB like activity of VSMCs were investigated by electrophoretic mobility shift assay(EMSA). Results:NF κB activation of VSMCs could be induced by H 2O 2 or new born calf serum(NCS) for 72 h, the gray measure of the protein bindings measured through computer scanning was 63.9 and 46 6 respectively. NF κB activity of VSMCs stimulated by H 2O 2 or NCS might be prevented by 100 μmol/L probucol, the inhibitory rate was 37.1%, 14.8% respectively. Conclusion:probucol can inhibit NF κB activity of VSMCs stimulated by NCS or H 2O 2. These effects of probucol might help to reduce development of atheroclerosis and restenosis after percutaneous transluminal coronary angioplasty. [

关 键 词：普罗布考 降脂药 主动脉平滑肌细胞 NF-KB 体外培养 

分 类 号：R972.6[医药卫生—药品]