硫酸软骨素对人肺癌A549细胞增殖及侵袭能力影响  

EFFECTS OF CHONDROITIN SULFATE ON PROLIFERATION AND INVASION OF A549 LUNG CANCER CELLS

作　　者：刘克为[1] 崔岩[2] 李春雨[3] 郭锡春[3] 高华[3] 

机构地区：[1]青岛大学医学院附属医院肿瘤科,山东青岛266003 [2]青岛大学医学院附属医院肾内科,山东青岛266003 [3]青岛大学医学院附属医院药学系,山东青岛266003

出　　处：《青岛大学医学院学报》2014年第2期109-111,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：青岛市科技支撑计划项目(11-2-3-1(7)-nsh))

摘　　要：目的探讨硫酸软骨素（CS）对人肺癌A549细胞增殖及侵袭能力的影响及可能的分子机制。方法常规方法培养人肺癌A549细胞，并给予含不同剂量CS的培养液培养，采用噻唑盐（MTT）法检测CS对A549细胞增殖的影响；采用流式细胞术（FCM）碘化丙啶染色法检测CS对A549细胞周期的影响；采用Transwell侵袭实验检测CS对A549细胞侵袭能力的影响。结果CS各浓度组干预A549细胞后，与对照组相比细胞生长均受到限制，差异具有显著性（F=10．39，q=6．45～17．48，P〈0．05）。24h细胞被抑制25％和50％时CS的浓度为6．6和28．4g／L。以7和30g／L的CS干预A549细胞24h后，FCM检测结果显示，与对照组相比，G0／G1期和G2／M期比例明显降低，S期比例明显升高，差异有显著性（F=16．29～38．01，q=6．15～16．38，P〈0．05）。Transwell侵袭实验显示，实验组A549细胞穿膜细胞数明显少于对照组，差异有显著性（F=3．19，q=4．89、15．38，P〈0．01）。结论CS对人肺癌A549细胞具有抑制增殖和侵袭的作用，其机制可能与抑制细胞分裂周期有关。Objective To investigate the effects and mechanism of chondroitin sulfate （CS） on proliferation and invasion of AS49 lung cancer cells. Methods AS49 cells were treated with CS at different concentrations. The effects of CS on AS49 cell proliferation were examined by Thiazole salt method （MTT）. The cell cycle of AS49 treated with CS was observed by FCM via Propidium iodide staining. The invasion of AS49 cells treated with CS was detected by Transwell assay. Results Compared with the control group, the proliferations of AS49 cells were inhibited significantly after treated with CS （F = 10.39, q = 6.4S -17.48, P 〈 0.05）. The concentration of CS was 6.6 and 28.4 giL when the cells were inhibited by 2S% and SO% after 24 h. The results of FCM showed that compared with the control group, the proportions of G0/G1 and G2/M were reduced significantly, the proportion of S phase was increased significantly after treated with 7 g /L and 30 g/L CS （F = 16.29 - 38.01 -q = 6.15 -16.38, P〈0.05）. The Transwell invasion showed that the numbers of cell-permeating septum in experimental group were significantly less than the control group （F=3.19;q=4.89,15.38;P〈0.05）. Conclusion CS suppresses the proliferation and invasion of AS49 cells, the possible mechanism is associated with the inhibition of cell division cycle.

关 键 词：软骨素硫酸盐类 人肺癌A549细胞 细胞增殖 肿瘤侵润 

分 类 号：R734.2[医药卫生—肿瘤]