Interaction of epicatechin with bovine serum albumin using fluorescence quenching combined with chemometrics  

作　　者：ZHAI Min WU HaiLong ZHANG ShuRong ZHANG XiHua SUN YanMei YU RuQin 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University

出　　处：《Science China Chemistry》2014年第5期748-754,共7页中国科学（化学英文版）

基　　金：financially supported by the National Natural Science Foundation of China(21175041);the National Basic Research Program of China(2012CB910602)

摘　　要：The interaction between BSA and epicatechin was studied using fluorescence quenching titrations combined with trilinear decomposition method and excitation-emission matrix(EEM)fluorescence.The resolved spectra were highly similar with the actual ones which indicated that the resolved results were reliable.The relevant parameters of the binding process were obtained by quantifying each substance in the complicated mixtures in situ.The quenching was static quenching,epicatechin had a weak interaction with BSA and the binding site was one.The total concentration and the free concentration of quenchers had different effect on the system.The results demonstrated that the method exploited in this article is a useful tool to investigate complicated interactions,avoiding complicated pretreatment and simplify experimental procedure.The interaction between BSA and epicatechin was studied using fluorescence quenching titrations combined with trilinear de- composition method and excitation-emission matrix （EEM） fluorescence. The resolved spectra were highly similar with the actual ones which indicated that the resolved results were reliable. The relevant parameters of the binding process were ob- tained by quantifying each substance in the complicated mixtures in situ. The quenching was static quenching, epicatechin had a weak interaction with BSA and the binding site was one. The total concentration and the free concentration of quenchers had different effect on the system. The results demonstrated that the method exploited in this article is a useful tool to investigate complicated interactions, avoiding complicated pretreatment and simplify experimental procedure.

关 键 词：EPICATECHIN bovine serum albumin three-dimensional fluorescence quenching trilinear decomposition method in situinterpretation of binding parameters 

分 类 号：O641.3[理学—物理化学]