μ型阿片肽受体对癫痫敏感大鼠海马NR2B表达的影响  

Effects of mu-opioid receptors on hippocampal NR2B expression in rats with seizure susceptibility

作　　者：刘辉[1] 杜卫[1] 王利萍[1] 苏凡凡[1] 李靖宇[1] 

机构地区：[1]解放军第210医院神经内科,辽宁大连116021

出　　处：《临床军医杂志》2014年第4期340-343,共4页Clinical Journal of Medical Officers

摘　　要：目的探讨μ型阿片肽受体(MORs)介导大鼠癫痫敏感性形成过程中海马NMDA2B受体(NR2B)表达变化。方法红藻氨酸(KA)皮下注射建立大鼠癫痫发作模型,采用脑立体定位及微渗透泵技术,海马内微量注射MORs激动剂PL017或和拮抗剂β-FNA。7 d后通过阈下剂量KA检测大鼠癫痫发作敏感性,应用原位杂交方法观察海马CA1区锥体细胞及齿状回颗粒细胞NR2B表达变化。结果 PL017可显著增加大鼠海马CA1区锥体细胞和齿状回颗粒细胞NR2B mRNA(+)神经元数及平均灰度。β-FNA则明显降低两个脑区的NR2B mRNA(+)神经元数及平均灰度。结论 MORs介导癫痫发作敏感性形成机制与NR2B表达上调有关。Objective To investigate hippocampal NMDA receptor 2B （NR2B） expression in the rats with seizure susceptibility mediated by mu-opioid receptors （MORs）. Methods The SD rats were injected subcutaneously with a convulsive dose （ 10 mg/kg） of kainic acid （KA）. Normal saline （NS） , selective MORs antagonistβ--FNA or selective MORs agonist PL0tTwere infused constantly into the ventral hippocampi for 7 days by mini-osmotic pumps. Seven days later, all the rats were given subcutaneously with subcon- vulsant dose of KA （5 mg/kg） to determine the situation of seizure susceptibility. Then the expression of NR2B mRNA in CA~ py- ramidal neurons and dentate gyms granule cells （DGCs） of hippocampus was examined by in situ hybridization. Results The num- ber of NR2B mRNA positive neurons of CA1 and DGCs was increased in KA ＋ PL017 group comparing with normal group and KA ＋ NS group, and the density of NR2B mRNA neurons was enhanced in KA ＋ PL017 group comparing with normal group. The number and the density of NR2B mRNA positive neurons of CA1 and DGCs were decreased significantly in KA ＋ IS-FNA group comparing with normal, KA ＋ NS and KA ＋ PLol7 groups. Conclusion It is indicated that the seizure susceptibility of rats mediated by MORs was related to the up-regulation of hippocampal NR2B.

关 键 词：癫痫发作敏感性 u型阿片肽受体 NMDA受体2B亚型 红藻氨酸 海马 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]