化学治疗药物对肝癌细胞增殖抑制作用的观察  

The inhibitory effect of chemotherapeutic drugs on the proliferation of Bel-7402 cells

作　　者：朱建平[1] 蔡国友[2] 沈子威[2] 

机构地区：[1]北京大学第三临床医学院外科,100083 [2]清华大学生物系生物膜与膜生物工程国家重点实验室

出　　处：《中华普通外科杂志》2001年第1期28-30,共3页Chinese Journal of General Surgery

摘　　要：目的　评价化学治疗药物 (下称化疗药 )在肝癌治疗中的作用和地位。方法　采用MTT法测定 5 Fu、顺铂和 4 PA对肝癌细胞增殖的抑制作用。结果　 (1) 5 Fu、顺铂两药分别单剂使用 ,在给药后 0 5h肝癌细胞增殖活跃 ,而 12h后肝癌细胞增殖均受到抑制 ;对氯苯乙酸在给药后0 5h肝癌细胞增殖即被抑制。 (2 )肝癌细胞抑制率随化疗药的作用时间延长而增加 ,48～ 72h达 5 0 %以上的抑制率。 (3)化疗药对肝癌细胞的作用早期 ,癌细胞的生长有返跳活跃阶段 ,但总体上癌细胞的生长被抑制。结论　连续用药可很好地抑制肝癌细胞增殖 ,提高恶性肿瘤化学药物治疗的效果。Objective [WT5”BZ] To evaluate the in vitro effect of chemotherapeutant on hepatic carcinoma.[WT5”HZ] Methods [WT5”BZ] The inhibitory effect of 5 Fu, DDP and 4 PA on Bel 7402 cells proliferation was studied by MTT colorimetry.[WT5”HZ]Results [WT5”BZ] (1) Bel 7402 was on an active proliferation by the treatment of 5 Fu and DDP for half an hour, and the proliferation inhibited after 12 hours; but the proliferation was inhibited by 4 PA after half an hour of treatment. (2) The inhibitory rates increase with time and were over 50% after 48～72 hours of treatment. (3) There was temporarily active proliferation of Bel 7402 cells in the early period of chemotherapy by 5 Fu and DDP. [WT5”HZ]Conclusion [WT5”BZ] Continuous chemotherapy is effective for the inhibition of Bel 7402 cells proliferation. [WT5”HZ]

关 键 词：化学药物 癌 肝细胞 细胞分裂 

分 类 号：R73-36[医药卫生—肿瘤]