热休克对心肌细胞中αB-晶状体蛋白移位及表达的影响  

Effects of heat shock pretreatment on the translocation and expression of αB-crystallin in cultured neonatal rat cardiomyocytes

作　　者：肖卫民[1] 袁开宇[1] 袁灿[1] 王慷慨[1] 尢家騄[1] 肖献忠[1] 

机构地区：[1]中南大学湘雅医学院病理生理学教研室,长沙410078

出　　处：《湖南医科大学学报》2001年第1期1-3,共3页Bulletin of Hunan Medical University

基　　金：国家自然科学基金资助项目!(编号 :3 9770 3 0 7)

摘　　要：采用热休克对新生大鼠心肌细胞进行处理 ,观察αB 晶状体蛋白在细胞内的分布及其表达变化。结果 :①Western blot显示热休克后胞浆中可溶性αB 晶状体蛋白迅速移位至细胞内的不溶性结构中 ,2h左右基本复位 ;②免疫双荧光细胞化学显示热休克导致αB 晶状体蛋白在细胞浆内发生分布变化 ,而HSC70从胞浆向胞核移位 ;③免疫电镜定量分析进一步证实αB 晶状体蛋白在热休克时主要移向Z线及核膜等细胞骨架部位 ;④Northern blot显示热休克诱导心肌细胞αB 晶状体蛋白mRNA的表达增加。热休克处理使αB 晶状体蛋白迅速移位至细胞骨架 ,表明其在心肌预适应早期相中可能发挥保护作用。而αB 晶状体蛋白的诱导表达 ,可能是心肌预适应延迟相保护的重要机制之一。The effects of heat shock pretreatment on translocation and expression of αB crystallin were evaluated in neonatal rat cardiomyocytes. Results: ① Western blot analysis demonstrated that cytosolic soluble αB crystallin rapidly translocated to insoluble intracellular structure at 10 or 30 minutes and returned to cytosolic soluble compartment at about 1～2?h after heat shock. ② Double staining of immunofluorescence showed that αB crystallin redistributed in cytosol after heat shock, while HSC70 translocated from cytosol to nucleus. ③ Quantitative analysis with immunoelectronic microscopy demonstrated αB crystallin translocated to cytoskeletal structure such as Z line and nuclear envelope after heat shock. ④ Northern blot showed that heat shock induced αB crystallin mRNA expression. The rapid translocation and increasing expression of αB crystallin after heat shock suggests that this small heat shock protein may play an important protective roles during the early and late phases of myocardial ischemic preconditioning. [

关 键 词：热休克蛋白 细胞培养 心肌缺血 缺血预适应 αB-晶状体蛋白移位 

分 类 号：R364[医药卫生—病理学]