脑源性神经营养因子在胰腺导管腺癌中的表达及其临床意义  

Expression of brain derived neurotrophic factor in pancreatic duct cancer and its clinical significance

作　　者：胡立威[1] 陈炯[1] 周杭城[1] 杨仁保[1] 陈龙江[1] 赵跃[1] 

机构地区：[1]安徽医科大学附属省立医院普外科,合肥230001

出　　处：《中华胰腺病杂志》2014年第2期77-80,共4页Chinese Journal of Pancreatology

基　　金：国家自然科学基金（81071985）;安徽省国际科技合作计划资助项目（1008010038）;安徽省科技攻关计划资助项目（07010302230）;安徽省科技攻关计划资助项目（11010402172）;安徽省年度重点科研项目（1301043013）

摘　　要：目的 探讨脑源性神经营养因子（BDNF）在胰腺导管腺癌中的表达及其临床意义.方法 采用免疫组化SP法检测46例胰腺导管腺癌、38例胰腺良性疾病及20例正常胰腺组织中的BDNF表达,应用蛋白质印迹法和RT-PCR法检测BDNF蛋白及mRNA表达量,并分析BDNF表达与胰腺导管腺癌临床病理参数的相关性.结果 胰腺导管腺癌组织中BDNF阳性表达率为52.2％ （24/46）,胰腺良性疾病组织为7.8％ （3/38）,而正常胰腺组织未见BDNF阳性表达.正常胰腺、胰腺良性疾病、胰腺癌组织中BDNF蛋白表达量分别为0.38±0.01、0.56±0.01、0.97±0.01,mRNA表达量分别为0.85±0.14、1.67±0.21、3.45±0.67,胰腺癌和胰腺良性疾病的表达量均显著高于正常胰腺,胰腺癌又显著高于胰腺良性疾病,差异均具有统计学意义（P值均＜0.01）.BDNF阳性表达与胰腺导管腺癌的神经浸润、淋巴结转移存在相关性（P值均＜0.05）,而与患者性别、年龄及肿瘤大小、部位、分化程度等无相关性.结论 BDNF参与胰腺癌的发生、发展,并可能与胰腺癌患者预后有关.Objective To determine the expression of brain-derived neurotrophic factor（BDNF） in pancreatic ductal adenocarcinoma and its clinical significance. Methods SP immunohistochemical staining method was used to detect the expression of BDNF in 46 cases of pancreatic ductal adenocarcinoma, 38 cases of benign pancreatic diseases and 20 cases of normal pancreatic tissue. Real time PCR and Western blot was used to detect the protein and mRNA expression levels. The relationship between BDNF expression and clinicopathological parameters of pancreatic cancer was determined. Results The positive expression rate of BDNF was 52.2% （24/46） in pancreatic ductal adenocarcinoma pancreatic ductal adenocarcinoma, 7.8% （3/38） in benign pancreatic diseases, and none of the normal pancreatic tissue was BDNF positive. The BDNF protein expression levels in pancreatic ductal adenocarcinoma, benign pancreatic diseases and normal pancreatic tissue were 0.38 ± 0.01, 0.56 ± 0.01, 0.97 ± 0.01, respectively, and the BDNF mRNA expression levels were 0.85 ± 0.14, 1.67 ± 0.21, 3.45± 0.67, respectively, and the expression levels in pancreatic ductal adenocarcinoma and benign pancreatic diseases were significantly higher than that in normal pancreatic tissue, while the expression level in pancreatic ductal adenocarcinoma was significantly higher than that in benign pancreatic diseases （P 〈 0.05）. Positive BDNF expression was correlated with nerve infiltration

关 键 词：胰腺肿瘤 脑源性神经营养因子 肿瘤转移 基因表达 

分 类 号：R735.9[医药卫生—肿瘤]