兔软组织VX2肿瘤模型在MR介导下行无水乙醇瘤内注射消融治疗的实验研究  

Establishment of Rabbit VX2 Soft Tissue Tumor Model and Treatment with Percutaneous Ethanol Inj ection under the Guidance of Magnetic Resonance Imaging

作　　者：兰为顺[1] 胡道予[1] 李震[1] 李维[1] 胡衫 王梓[1] 汤浩[1] 胡瑶[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院放射科,湖北武汉430030

出　　处：《实用放射学杂志》2014年第4期671-674,共4页Journal of Practical Radiology

摘　　要：目的：建立兔软组织 VX2肿瘤模型,并在 MR介导下行无水乙醇瘤内注射消融治疗,为后期功能性 MRI评价疗效奠定基础。方法15只健康新西兰大白兔,雌雄不限,体重2.5-3 kg,在其右侧后大腿内侧肌肉内注入 VX2肿瘤细胞悬浮液各约0.2 mL,在种植肿瘤后的第14天行常规MR扫描,观察肿瘤种植的成功率及肿瘤的形态、大小、信号特点等。扫描后的第2天再在MR介导下行无水乙醇瘤内注射消融治疗。利用T2 WI作为导引及监测手段,将带侧孔的MR兼容穿刺针刺入病灶中心,按肿瘤直径1 mL/cm的量向瘤内缓慢注射无水乙醇,对肿瘤进行消融治疗。观察术前、术后肿瘤的形态特征、信号特征和病理特征。结果15只兔右侧后大腿内侧种植肿瘤均成功。常规 MR扫描肿块呈类圆形,直径约3-4 cm,T1 WI 肿瘤呈等信号或稍低信号,肿瘤中心可见更低信号的坏死区。T2 WI肿瘤呈高信号,中心坏死区为更高信号。肿瘤周边见高信号的水肿带；15只兔均成功进行了MR介导下的无水乙醇瘤内注射消融术,术中注射无水乙醇平均3.5 mL。T2 WI能清晰地监测无水乙醇在瘤内的弥散和分布。病理检查示：术前肿块周边肿瘤细胞密集,排列呈巢状,肿瘤细胞核大而深染,部分可见核分裂象。肿块中心可见少许黄白色豆腐渣样坏死组织。术后10 d肿块周边肿瘤组织大部分凝固性坏死崩解,肿瘤坏死率约85%-90%,仅见少数残存的肿瘤细胞,肿块中心坏死范围较术前明显增大。结论兔软组织 VX2肿瘤模型的建立操作简单,成瘤率高,是适合用作评价肿瘤疗效的动物模型；MR T2 WI可以清晰地监测无水乙醇在瘤内的弥散和分布,是一种好的导引和监测肿瘤消融治疗的影像学方法。Objective Establish rabbit VX2 soft tissue tumor model,and treat it with percutaneous ethanol injection（PEI）under the guidance of magnetic resonance imaging.Make ready for the therapeutic evaluation with functional magnetic resonance imaging. Methods Fifteen healthy New Zealand white rabbits were included in this study.0.2 mL tumor tissue suspensions were injected into the rabbits’posterior limb.14 days later,all rabbits were underwent conventional MRI examination.PET were performed to all the tumors under the guidance of MRI in the next day of the examination.T2 WI was used as guidance and monitoring means.MR com-patible puncture needle with lateral hole was stabed into the lesion center,and inj ected anhydrous ethanol according to the volume of tumors’diameter （1 mL/cm ）slowly.the tumors signal characteristics,morphological feature and pathological feature were ob-served pre and post-operation.Results All of the 1 5 rabbits were established soft tissue tumor model successfully;the success rate is 100%.The tumors were oval or round,3-4 cm in diam.MRI scanning showed low signal on T1 WI and high signal on T2 WI be-fore PEI.PEI was performed to all the tumors under the guidance of MR successfully with 3.5 mL ethanol injected into the tumors in average.T2 WI could monitor the ethanol in dispersion and distribution within the tumors clearly.Histologically,tumors were composed of large,uniform,oval/round cells arranged in solid nests which was intensive in the periphery of tumors.Necrosis tissue was apparent in the center of the tumors.10 days after operation,most tissue in the periphery of tumors was coagulative necrosis , only a few tumor cells left.Ranges of necrosis in the tumors center were obviously increased compared with pre-operation.Conclusion Rabbit VX2 tumor of soft tissue model is suitable for the therapeutic evaluation of tumor .It is an animal model which has the characteristic of simple to operate and high rate of suc-cessful.MR T2 WI can monitor the ethanol in dispersion and distribution

关 键 词：动物模型 VX2 肿瘤 磁共振成像 介入性 经皮无水乙醇注射 

分 类 号：R738.6[医药卫生—肿瘤] R445.2[医药卫生—临床医学]