结直肠癌中NQO1蛋白表达的临床病理及预后意义  

Clinicopathological and prognostic significance of NQO1 protein expression in patients with colorectal carcinoma

作　　者：马跃[1] 林黎娟[1,2] 朴俊杰[1] 吕蕙馨 林贞花[1] 

机构地区：[1]延边大学医学院病理学教研室,延吉133002 [2]辽东学院医学院影像系,丹东118002

出　　处：《临床与实验病理学杂志》2014年第4期366-370,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(61371067);吉林省科技厅中青年科研创新团队领军人才项目(20130521017JH)

摘　　要：目的 探讨NQO1蛋白在结直肠癌（colorectal cancer, CRC）组织中的表达及意义。方法 应用免疫组化法检测192例CRC、44例癌旁组织以及28例结肠不典型增生中NQO1的表达,并结合临床生物学指标及生存时间进行统计学分析。结果 CRC中NQO1阳性率明显高于良性组织（P均〈0.01）; NQO1表达与CRC分化程度、是否发生转移、浆膜侵犯及临床分期密切相关（P均〈0.05）; 生存分析显示,NQO1高表达者无瘤生存率及5年生存期均明显低于低表达者（P均〈0.001）; 与临床分期联合分析显示,无论在早期组还是晚期组,NQO1高表达患者无瘤生存率及5年生存率均低于低表达患者,差异均有统计学意义（P〈0.01）; Cox回归模型分析表明NQO1高表达是CRC预后的独立风险因子（HR：1.398,95%CI：1.011～1.934,P=0.043）。结论 检测NQO1蛋白表达对CRC的诊断及预后评估有重要的临床病理学意义,有望成为CRC生物学标记中的新指标。Purpose To investigate the prognostic significance of NQO1 protein expression in colorectal carcinoma （CRC） patients. Methods 192 cases of primary CRC, 28 of colonic dysplasia, and 44 of adjacent non-tumor tissues were selected for immunohisto- chemical staining of NQO1 ,protein. Correlation between NQO1 overexpression and clinicopathologic characteristics, and the survival rates were calculated by the statistical methods. Results The strongly positive rate of NQOI protein in CRC was significantly higher than that in gastric dysplasia and adjacent non-tumor tissues （P 〈 0. 01, respectively）. NQO1 high-expression rate was positively cor- related with differentiation, serosal invasion, lymph node metastasis, and clinical stage （ P 〈 0. 05, respectively）. Survival curve showed that the disease-free survival and 5-year survival rates of the patients with high NQO1 expression were obviously shorter than those of patients with low NQO1 expression （P 〈 0. 001, respectively）. Further analysis showed that, high expression of NQO1 predic- ted the lower disease-free survival and 5-year survival rates in late-stage patients （P 〈 0. 01, respectively）. Importantly, NQO1 was an independent risk factor for the prognosis of CRC using Cox proportional hazards regression model （HR： 1. 398,95% CI： 1.011 1. 934, P = 0. 043）. Conclusions Detection of NQO1 protein expression in CRC has an important clinical significance, and it is ex- pected to become a new biomarker for CRC.

关 键 词：结直肠肿瘤 NQO1蛋白 生存分析 预后 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]