结直肠癌中Bmi-1、p14^(ARF)和Mdm2的表达及临床意义  

Expression of Bmi-1,p14^(ARF) and Mdm2 and their clinical significance in colorectal carcinoma

作　　者：姚冬颖[1] 朱晓燕[2] 

机构地区：[1]河北省邢台市人民医院病理科,邢台054031 [2]河北省邢台市人民医院肿瘤内科,邢台054031

出　　处：《临床与实验病理学杂志》2014年第4期379-382,共4页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的探讨Bmi-1、p14ARF和Mdm2在结直肠癌中的表达及其与各临床病理参数的关系。方法应用免疫组化EliVision两步法检测125例结直肠癌和20例正常结直肠组织中Bmi-1、p14ARF和Mdm2的表达。结果 (1)Bmi-1和Mdm2在结直肠癌中的阳性率(56.8%和62.4%)明显高于正常结直肠组织(20.0%和15.0%),p14ARF在结直肠癌中的阳性率(47.2%)明显低于正常结直肠组织(75.0%)(P<0.05)。(2)Bmi-1表达与浆膜侵犯、淋巴结转移、临床分期密切相关,p14ARF表达与浆膜侵犯、临床分期密切相关,Mdm2表达与组织分化程度、淋巴结转移、临床分期密切相关(P<0.05)。(3)在结直肠癌中,Bmi-1和Mdm2表达与p14ARF表达均呈负相关(P<0.05)。结论 Bmi-1、p14ARF和Mdm2的异常表达参与结直肠癌的发生、发展及转移,可作为评价结直肠癌生物学行为的参考指标,并为临床治疗提供新靶点。Purpose To explore the expression of Bmi-1, p14^ARF and Mdm2 in colorectal carcinoma and to find out their association with elinicopathologieal features. Methods The expression of Bmi-1, p14^ARF and Mdm2 was detected by immunohistochemistry in 125 cases of colorectal carcinoma and 20 cases of normal colorectal tissues. Results （ 1 ） The positive rates of Bmi-1 and Mdm2 were 56. 8% and 62.4% in colorectal carcinoma, respectively, which were much higher than that in normal colorectal tissues （20. 0% and 15.0% ）. The positive rates of p14^ARF was 47.2% in colorectal carcinoma, which was much lower than that 75.0% in normal eolorec- tal tissues （P 〈 0.05 ）. （2） The expression of Bmi-1 was significantly correlated with serosal invasion, lymph node metastasis and Dukes stage. The expression of p14^ARF was significantly correlated with serosal invasion and Dukes stage. The expression of Mdm2 was significantly correlated with histologic grade, lymph node metastasis and Dukes stage （ P 〈 0. 05 ）. （ 3 ） In colorectal carcinoma, the expression of Bmi-1 and Mdm2 was negatively correlated with p14^ARF, respectively （P 〈 0.05 ）. Conclusions Abnormal expression of Bmi-1, p14^ARF and Mdm2 is involved in the occurrence, development and metastasis of eoloreetal carcinoma, which could be as the reference factors in judgement of biological behavior and provide a new target for clinical treatment.

关 键 词：结直肠肿瘤 BMI-1 P14^ARF MDM2 免疫组织化学 

分 类 号：R735.3[医药卫生—肿瘤]