肝癌细胞系L2不同途径接种造模的比较  

Comparison of liver cancer models established by different injection routes of rat L2 hepatocellular carcinoma cells

作　　者：刘勇[1] 唐红[1] 罗霞[1] 阮思蓓[1] 张元[1] 唐明希[1] 

机构地区：[1]泸州医学院附属医院病理科,泸州646000

出　　处：《临床与实验病理学杂志》2014年第4期393-396,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：四川省教育厅重点项目(08ZA090);四川省科技厅应用基础项目(2010JY0130);泸州医学院博士启动基金(2011204);泸州医学院科研启动基金(2012-LG-03);泸州医学院自然科学基金(55)

摘　　要：目的比较大鼠肝癌细胞系L2肝、脾原位接种肝细胞癌(hepatocellular carcinoma,HCC)发生及侵袭转移情况,筛选理想的L2 HCC肺转移动物模型。方法将20只7周龄雌性BALB/cA裸鼠随机分为2组,应用肝癌细胞系L2分别对2组小鼠进行肝、脾原位接种造模,观察裸鼠存活率,HCC的发生与侵袭转移率以及肿瘤病理学特点。结果肝原位接种组肝成瘤率100%,肿瘤呈结节膨胀性生长,与周围肝组织和腹壁侵袭粘连明显,肺转移率为60%,未见脾转移;脾原位接种组脾成瘤率78%,肝转移率67%,肿瘤亦呈结节膨胀性生长,与周围组织和腹壁也有侵袭粘连,肺转移率11%,明显低于肝接种组(P<0.05)。镜下肺门淋巴结转移率为33%。结论肝癌细胞系L2肝、脾原位接种均易成瘤且有肺转移发生,其中肝原位接种动物模型更适用于HCC肺转移机制及体内药物干预等实验分析。Purpose To compare and investigate the oncogenic and metastatic phenotypes in nude mice by injection of rat hepatocellu- lar carcinoma （HCC） ceils L2 via two different mutes. Methods Twenty of 7-week-old female BALB/cA mice were randomly divided into 2 groups. After the injections of L2 from liver or spleen lobe, the survival rate, tumor formation rate, carcinogenic features, and metastasis were comparatively studied. Results All of the liver orthotopic nude mice developed liver cancer （ 100% ） with 60% lung metastasis rate, and exhibited an expansive growing pattern with surrounding invasion. In the spleen orthotopic model, 78% mice de- veloped HCC in spleen, with 67% liver metastatic rate and 11% lung metastatic rate, lower than the liver orthotopic model （P 〈 0. 05）. But the microscopically hilar lymph node metastasis rate was 33%. Conclusion The direct liver injection of L2 in female nude mice is a better modeling method for studying the mechanism of both carcinogenesis and metastasis, as well as the evaluation of therapeutic effect of liver cancer.

关 键 词：肝肿瘤 肝癌细胞系L2 转移 裸鼠 动物模型 

分 类 号：R735.7[医药卫生—肿瘤] R33[医药卫生—临床医学]