Micro CT应用于犬即刻种植即刻加载后种植体-骨界面骨结合的研究  

The osseointegration research of immediate loading of immediate implant on implant-bone interface in dogs by using Micro CT

作　　者：张亨国[1] 刘向辉[1] 孙卫革[1] 张磊[1] 沈彬[1] 叶长林[1] 

机构地区：[1]安徽医科大学解放军八一临床学院(中国人民解放军第八一医院)口腔科,南京210002

出　　处：《安徽医科大学学报》2014年第4期455-459,共5页Acta Universitatis Medicinalis Anhui

基　　金：2012年度南京军区医学科技创新课题项目面上A类(编号:12MA040)

摘　　要：目的应用Micro CT对即刻种植即刻加载后种植体-骨界面骨结合情况进行研究。方法 4只Beagle犬,拔除其双侧下颌第3、4前磨牙,在相应的拔牙窝选择最佳位置即刻植入DIO种植体(直径3.8 mm,长度10.0 mm)各1颗,同期连接基台(直径3.8 mm,长度6.0 mm)。术后24 h内采用种植体垂直加载仪进行垂直加载,将每只犬下颌的4枚种植体按照加载力的不同分为:对照组(不加载),加载5、10、15N组,加载频率为2 Hz,加载时间为每天15 min。开始加载后第4、8周后各处死2只Beagle犬,制作离体犬下颌骨标本并用Micro CT扫描,以CTAn软件分析扫描后图像,取与种植体相邻的感兴趣区行骨密度(BMD)及骨计量学分析。结果①加载4周时,加载10 N组与加载5、15 N组以及对照组在BMD、骨小梁数目、厚度、形态等方面差异均有统计学意义(P<0.05)。②加载8周时,加载10 N组与加载5、15N组以及对照组在骨小梁数目、厚度、形态等方面差异均有统计学意义(P<0.05)。③加载8周时,在骨小梁数目、厚度、形态等方面,加载15 N组与加载5、10 N组及对照组差异均有统计学意义(P<0.05)。结论应用Micro CT对种植体-骨界面新生骨骨小梁情况进行分析,客观地对不同加载时期不同加载项下种植体-骨界面骨结合情况进行了评估,为牙即刻种植术及术后即刻加载提供了理论依据。Objective The osseointegration research of immediate loading of immediate implant on implant-bone interface by using Micro CT. Methods Four Beagle dogs were used. The bilateral mandibular third and fourth premolars were extracted, meanwhile DIO implants （3.8 mm in diameter and 10.0 mm in length） and DIO abut- ments （3.8 mm in diameter and 6.0 mm in length） were immediately implanted in the corresponding tooth fossa of each dog. A technical device was applied to the vertical loading of the immediate implants at 24 h after the opera- tion. The implants were divided into four groups including a 5 N group, a 10 N group, a 15 N group and a control group according to the load. Each group was loaded with 2 Hz frequency for 15 rain everyday. Two dogs were sacri- ficed at 4 weeks and two dogs at 8 weeks to prepare the mandibular specimens in vitro. Many images were generated by Micro CT which scanned the mandibular specimens. After software CTAn was used to analyze the scan images, chose the interest regions adjacent to implant and analysed the bone mineral density and microstructure measure- ment. Results Compared 4 weeks with 8 weeks on each loading force, BMD, BV/TV, Tb. Th, Tb. N, Tb. Pf, SMI, Po all resulted in significant difference （P 〈 0.05 ）. Compared 10 N group with 5 N group, 15 N group and control group at 4 weeks or 8 weeks, BMD, BV/TV, Tb. Th, Tb. N, Tb. Pf, SMI, Po all resulted in significant difference （P 〈0. 05）. Compared 15 N group with 5 N group,10 N group and control group at 8 weeks, BMD, BV/TV, Tb. Th, Tb. N, Tb. Pf, SMI, Po all resulted in significant difference（P 〈 0. 05 ）. Conclusion The anal- ysis of bone trabecula on implant-bone interface worked by Micro CT could assess the osseointegration of different loading force and loading period more objectively, which provides theoretical foundation for immediate implanting and immediate loading.

关 键 词：MICRO CT 即刻种植 即刻加载 种植体骨界面 骨结合 

分 类 号：R783.4[医药卫生—口腔医学] R318.01[医药卫生—临床医学]