昆虫几丁质酶的分类、功能分析、重组表达及酶学性质的研究进展  

Research Progress of Classification, Functional Analysis, Recombinant Expression and Enzymology Properties on Insect Chitinase

作　　者：任慧[1] 张常[1] 范晓军[1] 

机构地区：[1]太原理工大学化学化工学院生物与制药工程系,太原030024

出　　处：《农业生物技术学报》2014年第4期502-507,共6页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.31101490);山西省青年科技研究基金(No.2010021031-2);太原市科技项目(No.120164087)

摘　　要：昆虫几丁质酶及其类似蛋白在昆虫生长和发育阶段起到重要调节作用。最近几年,运用新的生物技术对昆虫几丁质酶的结构,时空表达,和组织特异性表达以及生物学功能进行了深入研究,取得了许多新的成果。本文就昆虫几丁质酶基因的结构特征,几丁质酶生理功能、重组表达特点、酶活测定方法及影响酶活性因素等方面进行讨论与综述。In the insect growth and development stages, insect chitinase and chitinase-like proteins play an important regulatory role. In recent years, new technologies were applied for the structure of insect chitinase, time and space expression, tissue specific expression and biological function of insect chitinase, and got many new research achievements. In this paper, the structure of insect chitinases classification, physiological function, recombinant expression, methods of detection of enzyme activity and influencing factors of enzyme activity and other aspects are discussed and reviewed.

关 键 词：昆虫几丁质酶 结构分类 功能分析 重组表达 酶学性质 

分 类 号：S816.7[农业科学—饲料科学]