波氏假丝酵母启动子的克隆及新霉素基因的表达  

Cloning of Promoter from Candida boidinii and the Expression of Neomycin Resistant Gene

作　　者：徐柏年[1] 吴江雪[1] 李文清[1] 罗进贤[1] 

机构地区：[1]中山大学生命科学学院/生物防治国家重点实验室,广东广州510275

出　　处：《中山大学学报（自然科学版）》2001年第1期65-68,76,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省自然科学基金项目资助项目(970175)

摘　　要：报道了波氏假丝酵母（Ｃａｎｄｉｄａ ｂｏｉｄｉｎｉｉ）启动子的克隆，将克隆的启动子与 Ｎｅｏ基因重组构建了 含Ｎｅｏｒ基因的表达载体ＹｅｐＮＰ１８１，转化波氏假丝酵母，实现了Ｎｅｏｒ基因在波氏假丝酵母中的表达，使波氏 假丝酵母转化子能在含Ｇ４１８的抗性培养基上生长从而建立了Ｎｅｏｒ基因──Ｇ４１８选择系统，为外源基因在 波氏假丝酵母中表达创造了条件．Candida boidinii is one of the methylotrophic yeast with good development and application perspective hut very little study has been done as a gene expression system. The C. boidinii promoter was cloned and the ex-pression vector YepNP181 was constructed by recombining the cloned promoter and Neor gene onto an E. coli -yeast shuttle vector. YEpNP181 was then transformed into C. boidinii and Neor gene on YEpNP181 expressed. The transformants grow on YPD plate containing 300 μg/mL C418 and Neor-G418 selection system was thus established, which facilitates the foreign gene expression in C. boidinii.

关 键 词：启动子克隆 新霉素抗性基因 波氏假丝酵母 基因表达 工业发酵菌种 G418选择系统 转化子 

分 类 号：Q949.326.1[生物学—植物学] Q78