Caveolin-2在内皮-单核细胞激活多肽-Ⅱ增强血肿瘤屏障通透性中的表达水平变化  

Changes in the expression level of caveolin-2 in endothelial monocyte activating polypeptide-II-induced increase in blood-tumor barrier permeability

作　　者：李振[1] 刘云会[1] 薛一雪[2] 王萍[2] 刘丽波[2] 

机构地区：[1]中国医科大学附属盛京医院神经外科,辽宁沈阳110004 [2]中国医科大学基础医学院神经生物学教研室,辽宁沈阳110001

出　　处：《现代肿瘤医学》2014年第4期742-746,共5页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81272564;81272795;81372484);高等学校博士学科点专项科研基金资助项目(2009210411 0015;20102104110009);沈阳市科学技术计划项目(F13-220-9-15;F13-316-1-16;F13-316-1-19)

摘　　要：目的:检测质膜微囊结构蛋白caveolin-2在内皮-单核细胞激活多肽-II(EMAP-II)增强血肿瘤屏障(BTB)通透性过程中的表达水平变化。方法:荷瘤Wistar大鼠被随机分成4组(每组16只):EMAP-II处理0h、1h、2h和4h组。采用伊文思蓝渗透性实验评估各组BTB通透性变化情况;RT-PCR、Western blotting、免疫组化和免疫荧光法检测脑胶质瘤组织毛细血管上caveolin-2的表达水平变化。结果:EMAP-II作用1h时,BTB的通透性显著增强,随后逐渐降低,4h时恢复正常;同时,EMAP-II作用1h时,脑胶质瘤组织毛细血管上caveolin-2的表达水平显著增高,随后逐渐降低,4h时恢复正常。结论:EMAP-II可能通过caveolae介导的跨细胞途径来增强BTB的通透性,其机制与caveolin-2的表达水平上调有关。Objective： To detect changes in the expression level of caveolar structural protein caveolin - 2 in endo- thelial monocyte activating polypeptide- II（ EMAP -II ） -induced increase in blood -tumor barrier（ BTB ）permea- bility. Methods：Tumor- bearing rats were randomly divided into 4 groups（ n = 16, each） ： EMAP- II Oh, l h ,2h and 4h groups. Evans blue method was used to evaluate the alterations in BTB permeability. Changes in the expression lev- el of the caveolar structural protein caveolin - 2 in brain capillaries were measured by RT - PCR, Western blotting, immunohistochemistry and immunofluorescent assays. Results：The EB content in tumor tissues increased significantly at 1 h after EMAP - II administration, and trended back toward the level of the EMAP - II Oh group gradually by 4h. The expression levels of caveolin -2 in brain capillaries were significantly increased at lh, and decreased gradually toward the untreated level thereafter. Conclusion： EMAP - II may induce BTB hyperpermeability via caveolae - medi- ated transcellular pathway, which is associated with the upregulation of caveolin - 2.

关 键 词：内皮一单核细胞激活多肽-II 血肿瘤屏障 通透性 caveolin-2 跨细胞途径 

分 类 号：R730[医药卫生—肿瘤] R739.41[医药卫生—临床医学]