CXCL12对人乳腺癌细胞株MDA-MB-435失巢凋亡的研究  

The effect of CXCL12 on anoikis in human breast cancer cell line MDA-MB-435

作　　者：杨开[1] 杨越[1] 曹翌[1] 王政华[2] 

机构地区：[1]锦州石化公司医院普通外科,辽宁锦州121000 [2]辽宁医学院附属第一医院肿瘤科,辽宁锦州121000

出　　处：《现代肿瘤医学》2014年第4期769-772,共4页Journal of Modern Oncology

基　　金：辽宁省教育厅科学技术项目(NO:L2010283)

摘　　要：目的:研究趋化因子CXCL12对人乳腺癌高转移细胞系MDA-MB-435失巢凋亡的影响。方法:实验组培养基中加入终浓度为50ng/ml的CXCL12,对照组为无CXCL12的DMEM培养基。两组细胞悬浮培养,建立人乳腺癌高转移细胞系MDA-MB-435失巢凋亡抵抗细胞模型。MTT检测CXCL12对于失巢凋亡抵抗MDA-MB-435细胞系生长的影响,流式细胞仪检测两组细胞的凋亡情况,Transwell实验检测细胞侵袭能力改变。结果:CXCL12对于失巢凋亡抵抗MDA-MB-435细胞系在形态学方面与对照组相比无特异性改变。CXCL12作用组失巢凋亡抵抗细胞增殖能力低于对照组,凋亡率增加,侵袭能力增加,穿过膜细胞数明显高于对照组(28±3.0 vs 15±2.4,P<0.05)。结论:在人乳腺癌高转移细胞系MDA-MB-435失巢凋亡过程中,CXCL12能在一定程度上抑制失巢凋亡抵抗细胞的生长,但却能增加存活肿瘤细胞的侵袭性。Objective：To investigate the effect of chemokine CXCL12 on anoikis in human high -metastatic breast cancer cell line MDA - MB - 435. Methods ： MDA - MB - 435 was treated with CXCL12 at the concentration of 50ng/ml in experimental group, MDA - MB -435 was treated with DMEM without CXCL12 in control group. We per- formed suspension technology to culture MDA - MB -435 to establish anoikis resistant cells. Further to investigate the proliferation of MDA - MB -435 by MTT and the apoptosis with FAS treated with CXCL12. The cancer cell invasive ability was assessed by Transwell. Results ： Anoikis - resistant MDA - MB - 435 cells treated with CXCL12 had the same pathology with the control group. The treatment of CXCL12 down - regulated the proliferation of anoikis - resist- ant MDA- MB- 435 cells accompanied with apoptosis, up- regulated the invasive ability, the number of migration cells of hypoxia was higher than in control group （28± 3.0 vs 15 ± 2.4, P 〈 0.05 ）. Conclusion： In the anoikis - re- sistance of human high - metastatic breast cancer cell line MDA - MB -435, CXCL12 inhibited the survival of anoikis resistant cells and up -regulated the survival cell invasive ability.

关 键 词：乳腺癌 MDA-MB-435 CXCL12 失巢凋亡 

分 类 号：R737.9[医药卫生—肿瘤]