单核细胞与肺癌H460细胞及肺成纤维细胞三维立体混合培养IL-1β与TNF-α的表达  

The expression of IL-1β and TNF-α in co-culture of mononuclear cells,H460 and fetal lung fibroblast in three-dimensional culture model

作　　者：杨娜[1] 陈卫强[1] 李继东[1] 朱运奎[1] 

机构地区：[1]兰州军区兰州总医院,甘肃兰州730050

出　　处：《现代肿瘤医学》2014年第4期787-789,共3页Journal of Modern Oncology

基　　金：甘肃省自然科学研究基金资助项目(编号:SZS051-A25-088)

摘　　要：目的:探讨外周血单核细胞和肺癌H460细胞及肺成纤维细胞相互作用对产生IL-1β与TNF-α的影响。方法:三维胶原立体培养条件下,分为单核细胞、H460细胞、肺成纤维细胞单独培养组,单核细胞和H460细胞共同培养组,单核细胞和肺成纤维细胞共同培养组,H460细胞和肺成纤维细胞共同培养组,单核细胞、H460细胞和肺成纤维细胞共同培养组。培养24、72、96h,收集上清液,用酶联免疫法检测不同时间点各组IL-1β与TNF-α的含量。结果:各组细胞在三维立体胶原培养条件下生长良好,单独培养时,仅单核细胞组分泌少量IL-1β和TNF-α,而H460细胞组、肺成纤维细胞组没有检测到IL-1β和TNF-α。单核细胞和H460细胞共同培养组分泌IL-1β与TNF-α量增高,单核细胞和肺成纤维细胞共同培养组分泌IL-1β与TNF-α量也有轻微增高,而H460细胞和肺成纤维细胞共同培养组仅检测到少量IL-1β与TNF-α的表达,单核细胞、H460细胞和肺成纤维细胞共同培养组分泌IL-1β与TNF-α量明显高于其它组。结论:单核细胞和肺癌H460细胞及肺成纤维细胞相互作用可以促进IL-1β与TNF-α的表达。Objective： To explore the interactions among mononuclear cells, H460 and fetal lung fibroblast and the influence on the expression of IL- 1B and TNF-ct in three- dimensional culture model. Methods：Cells cultured in 3D collagen gels were divided into H460 group （HG）, mononuclear cells group（MG）, fetal lung fibroblast group （ HFLG）, co - culture groups of mononuclear and H460, mononuclear and fetal lung fibroblast, H460 and fetal lung fi- broblast, mononuclear cells and H460 and fetal lung fibroblast. The expressions of IL - 1B and TNF - a were detected by ELISA at 24,72,96h after culture. Results：Cells cultured in three - dimensional culture collagen gels were well, IL - 1B and TNF - a were secreted slightly in MG, and IL - 1B and TNF - of were not detected in HG and HFLG. The levels of IL - 1B and TNF - a enhanced in co - culture groups of mononuclear cells and H460, and also enhanced slightly in co - cultured mononuclear cells and fetal lung fibroblast. Co - cultures of H460 with fetal lung fibroblast re- sultured little IL - 1B and TNF - a ; when co - culture mononuclear cells and I-I460 and fetal lung fibroblast, the levels of IL - 1B and TNF - a were significantly higher than the other groups. Conclusion： More IL - 1B and TNF - a se- creted in co - culture mononuclear cells and I-t460 and fetal lung fibroblast.

关 键 词：单核细胞 肺癌H460细胞 肺成纤维细胞 肺癌 侵袭 转移 

分 类 号：R734.2[医药卫生—肿瘤]