皮肤鳞癌中DBPA mRNA的表达及其与表皮分化的关系  

Expression of DBPA in skin squamous cell carcinoma and the relationship to epidermal differentiation

作　　者：郑焱[1] 来宝长[2] 王秀莹[1] 田琼[1] 董昕煜 罗素菊[3] 刑立亚 李政霄[1] 肖生祥[1] 

机构地区：[1]西安交通大学医学院附属第二医院皮肤科,陕西西安710004 [2]西安交通大学医学院,陕西西安710061 [3]天津医科大学总医院皮肤科,天津300052 [4]西安市第一医院皮肤科,陕西西安710002

出　　处：《现代肿瘤医学》2014年第4期900-902,共3页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(编号:81071299;81371 732);教育部新世纪优秀人才支持计划;中央高校基本科研业务费

摘　　要：目的:DNA结合蛋白(DBPA)又称为ZO-1相关核苷酸结合蛋白(ZONAB),有很重要的转录活性,ZONAB可与ZO-1相结合,并通过抑制G1-S期的转化来调节上皮的增殖。本文研究DBPA在皮肤鳞癌中的作用机制及其与角质形成细胞异常分化的关系。方法:用原位杂交的方法检测正常皮肤组织、未受累组织及鳞癌皮损中DBPA mRNA的表达变化。结果:DBPA mRNA强表达于正常组织表皮颗粒层,弱表达于棘层上部;在未受累组织中强表达于颗粒层及棘层上部;在鳞癌中,DBPA可表达于绝大多数的肿瘤细胞。正常组织和未受累组织中DBPA的表达均与鳞癌皮损中的表达有显著差异性(P<0.01)。结论:DBPA可能参与鳞癌的角质形成细胞的分化和增殖。Objective： DBPA （ DNA - binding protein A） also called ZONAB [ zonula occludens 1 （ ZO - 1 ） - asso- ciated nucleic -acidbinding protein] is a Y -box transcription factor that regulates epithelial proliferation by restric- ting G~ - S phase progression binding to ZO - 1. This article will investigate the expression of DBPA in the pathogene- sis of skin squamous cell carcinoma（SCC）. Methods：The levels of DBPA mRNA in normal skin tissues, uninvolved skin and SCC lesions were determined by in situ hybridization. Results：DBPA mRNA was strongly expressed in gran- ular layers and slightly expressed in upper layers of stratum spinosum, in uninvolved epidermis it was strongly ex- pressed in granular layers and upper layers of stratum spinosum, while in SCC, it was strongly detected in nearly all tumor cells. There~ significant difference between normal tissues, uninvolved tissues and the lesions from SCC. Con- clusion：DBPA may implicated keratinocyte differentiation and proliferation in SCC.

关 键 词：鳞癌 DBPA 分化蛋白 

分 类 号：R739.5[医药卫生—肿瘤]