MCI-186对痴呆模型大鼠认知损害和脑内DNA氧化应激的影响  

Effects of MCI-186 on cognitive impairment and DNA oxidative stress in rat model of dementia

作　　者：周珊珊[1] 王欣[1] 盛莉[1] 黄丹华[2] 迟立君[1] 张黎明[1] 

机构地区：[1]哈尔滨医科大学附属第一医院神经内科,黑龙江省哈尔滨市150001 [2]齐齐哈尔医学院附属第三医院神经内科,黑龙江省齐齐哈尔市161000

出　　处：《国际神经病学神经外科学杂志》2014年第1期7-11,共5页Journal of International Neurology and Neurosurgery

基　　金：黑龙江省教育厅科学技术研究项目(12531303)

摘　　要：目的观察MCI-186对双侧脑室内注射链脲菌素(STZ)诱导的认知损害模型大鼠认知功能和脑内DNA氧化应激的影响。方法 64只成年雄性SD大鼠随机分为4组:假手术组(S)、MCI-186+假手术组(M+S)、模型组(L)和MCI-186+制模组(M+L)。跳台试验和水迷宫试验测试大鼠认知功能;比色法和免疫荧光法分别检测大鼠脑内的H2O2、CAT含量和8OHdG表达。结果跳台试验中,与S组比较,L组潜伏期(179.1±41.3)显著缩短(P<0.05),错误次数(3.88±0.87)显著增加(P<0.05);与L组比较,M+L组潜伏期(250.5±27.1)显著延长(P<0.05),错误次数(2.83±0.64)显著减少(P<0.05)。Morris水迷宫试验中,与S组比较,L组大鼠潜伏期(36.1±3.59)显著延长(P<0.05);与L组比较,M+L组潜伏期(18.63±3.73)显著缩短(P<0.05)。与L组比较,M+L组脑内H2O2含量显著减少(P<0.05),CAT活性显著提高(P<0.05),8 OHdG表达阳性率显著降低(P<0.05)。结论 MCI-186能够改善STZ诱导的大鼠认知损害和脑内DNA氧化应激。Objective To observe the effects of MCI-186 on the cognitive function and DNA oxidative stress in the rat model of cogni- tive impairment induced by bilateral intracerebroventricular （ICV） injection of streptozotocin （STZ）. Methods Sixty-four adult male Sprague-Dawley rats were randomly divided into 4 groups： Sham （S） , MCI-186 ＋ Sham （M ＋ S）, ICV-STZ Lesion （L）, and MCI- 186 ＋ Lesion （ M ＋ L）. The step-down test was used to evaluate potential deficits in memory retention. Spatial learning and memory were tested using the Morris water maze （MWM）. The content of hydrogen peroxide （H2O2 ） and activity of catalase （CAT） in the cerebral cortex and hippocampal neurons were determined by calorimetric method. Immunofluorescence staining was used to measure the expression of 80HdG. Results In the step-down test, compared with the S group, the L group had a significantly reduced latency （ 179.1 ±41.3） but a significantly increased number of errors （3.88 ±0.87） （P 〈0.05 for both） ; compared with the L group, the M ＋ L group had a significantly increased latency （ 250.5 ±27.1 ） but a significantly reduced number of errors （ 2.83 ±0.64 ） （ P 〈 0.05 for both）. The MWM test showed that the L group had a significantly longer latency （36.1 ±3.59） than the S group （ P 〈 0.05 ）, and the M ＋ L group had a significantly shorter latency （ 18.63 ± 3.73 ） than the L group （P 〈 0.05）. Compared with the L group, the M ＋ L group had a significantly reduced content of H2O2 （ P 〈 0.05 ）, a significantly increased activity of CAT （P 〈 0.05 ）, and a signifi- cantly decreased positive rate of 8OHdG expression （P 〈 0.05 ）. Conclusions MCI-186 can reduce the STZ-induced cognitive impair- ment and DNA oxidative stress in rats.

关 键 词：MCI-186 痴呆 认知损害 DNA 氧化应激 

分 类 号：R749.16[医药卫生—神经病学与精神病学]