氯化1-辛基-3-甲基咪唑对EMT6细胞的毒性及其DNA损伤作用  

Cytotoxicity and DNA Damage Caused by 1-octyl-3-methylimidazolium Chloride on EMT6 Cells

作　　者：井长勤[1] 刘振华[1] 王燕[1] 李小英[1] 齐博[2] 刘中何[2] 于毅[1] 栗炳南[1] 丰慧根[1] 

机构地区：[1]新乡医学院生命科学技术学院,河南新乡453003 [2]新乡医学院第一附属医院,河南卫辉453100

出　　处：《河南师范大学学报（自然科学版）》2014年第2期124-130,共7页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省科技攻关重点项目(122102310195)

摘　　要：研究了氯化1-辛基-3-甲基咪唑([C8mim][Cl])对EMT6细胞的毒性大小及其可能的遗传机制.不同浓度(0.06、0.25、1.00mmol·L-1)的[C8mim][Cl]对EMT6细胞染毒12h,WST-1比色法检测了细胞活力,ELISA方法检测了Bcl-2蛋白的表达,Hoechst 33342染色检测了细胞核形态的变化,单细胞凝胶电泳(SCGE)检测了细胞DNA的损伤,流式细胞仪检测了细胞周期和细胞DNA的含量.结果显示,经[C8mim][Cl]染毒后,EMT6细胞活力下降,并且呈剂量依赖关系.当[C8mim][Cl]浓度高于0.25mmol·L-1时,与对照相比,差异显著.[C8mim][Cl]染毒使Bcl-2蛋白表达下调,引起了细胞核形态改变,造成了DNA的断裂损伤和含量下降,诱导了细胞凋亡.从而可以得出结论,DNA损伤参与了[C8mim][Cl]诱导的细胞凋亡.Cytotoxicity and underlying genetic mechanisms of 1 octyl-3 methylimidazolium chloride （[C8 mim] [CI1] on EMT6 cells were studied in the present research. EMT6 cells were exposed to [C8mim][C1] at the concentrations of 0.06, 0. 25, 1.00 mmol· L^-1 for 12 h, followed by evaluations of WST-1 assay, expression level of Bcl-2 by ELISA, nuclear morpho- logical alternation by Hoechst 33342 staining, DNA damage by single cell gel eleetrophoresis （SCGE）, and cell cycle and DNA contents by flow cytometry respectively. The results demonstrated that [C8 mim][C1] inhibits EMT6 cell growth and decreases their viabilities in a dose-dependent manner after 12 h of exposure. Compared to the control group, the difference is significant when the concentrations of [C8mim][C1] are higher than 0. 25 mmol · L^- 1. Our results also reveal that [C8mim][C1] can down-regulate Bcl-2 expression, causes nuclear morphological changes and DNA damage, and induces cell apoptosis. These resuits suggest that DNA damage may be involved in the apoptosis induced by [C8mim][Cl] in EMT6 ceils.

关 键 词：离子液体 EMT6细胞 细胞毒性 凋亡 DNA损伤 

分 类 号：X171.5[环境科学与工程—环境科学]