人抗原R与乏氧诱导因子1α在肾细胞癌中的表达及临床意义  

Expression and clinical significance of human antigen R and hypoxia inducible factor 1 alpha in renal cell carcinoma

作　　者：张志刚[2] 叶烈夫[1] 林乐[1] 朱庆国[1] 何延瑜[1] 李涛[1] 杨风光[1] 陈新[3] 黄水通[1] 胡敏雄[1] 叶世华[1] 陈松茂[1] 陈如[1] 

机构地区：[1]福建医科大学省立临床学院福建省立医院泌尿外科,福州350001 [2]西安医学院第二附属医院泌尿外科 [3]福建医科大学省立临床学院福建省立医院病理科,福州350001

出　　处：《中华泌尿外科杂志》2014年第4期262-266,共5页Chinese Journal of Urology

基　　金：福建省卫生厅医学创新课题（2009-CXB-10）

摘　　要：目的 探讨人抗原R（HuR）与乏氧诱导因子1α（HIF-1α）在肾细胞癌中表达的意义及二者的相关性. 方法 应用免疫组化PV-9000两步法检测2007年1月至2012年7月76例肾癌组织、20例癌旁组织、15例正常肾组织中HuR蛋白及HIF-1α蛋白的表达情况,分析HuR及HIF-1α蛋白表达与肾癌临床病理参数的关系,评价两者在肾癌中表达的相关性.Kaplan-Meier法进行生存分析,采用Spearman等级相关分析、X2检验等进行相关性分析. 结果 HuR在肾癌细胞质中的阳性表达率（38.2％）明显高于癌旁组织（10.0％）和正常肾组织（13.3％）,差异有统计学意义（P＜0.05）;HIF-1α在肾癌胞核中的阳性表达率（48.7％）明显高于癌旁组织（5.0％）和正常肾组织（0）,差异有统计学意义（P＜0.05）.胞质HuR表达水平与肾癌临床分期、病理类型及淋巴结转移有相关性（P＜0.05）;胞核HIF-1α表达水平与肾癌临床分期、病理分级和淋巴结转移有相关性（P＜0.05）.肾癌中胞质HuR表达水平与胞核HIF-1α表达水平呈正相关（P＜0.01）.66例肾癌患者随访6～ 67个月,HuR胞质阴性组及HIF-1α胞核阴性组的总体生存率及平均生存时间明显优于阳性组（P＜0.05）.结论 肾细胞癌组织中HuR及HIF-1α的表达水平和患者的临床病理参数及预后相关,二者有望成为肾癌诊断及预后评估的重要标志物.Objective To investigate the clinical significance and correlation of human antigen R （HuR） and hypoxia inducible factor 1 alpha （HIF-1α） expressions in renal cell carcinoma （RCC）.Methods Immuno-histochemistry PV9000 method was used to detect the expression of HuR and HIF-1α in 76 cases of RCC tissues,20 cases of para-carcinoma tissues and 15 cases of normal renal tissues.The correlation between the two proteins and clinical pathological parameters of RCC patients were analyzed.KaplanMeier estimates were used for survival analysis,and correlation analyses were conducted by using Spearman rank correlation method and chi-square test.Results The cytoplasm positive expression rate （38.2％） of HuR in RCC was higher than that in para-carcinoma tissues （10.0％） and normal renal tissues （13.3％,P＜0.05）.The nucleus positive expression rate （48.7％） of HIF-1α in RCC was significantly higher than that in para-carcinoma tissues （5.0％） and normal renal tissues （0,P＜0.05）.The expression level of HuR in cytoplasm was significantly correlated with the clinical stage,histological subtype and lymph node metastasis status （P＜0.05） in RCC.There was significant correlation between the expression level of nucleus HIF-1α and clinical stage,pathological grade and lymph node metastasis status in RCC （P＜0.05）.There was positive correlation between cytoplasm HuR and nucleus HIF-1α expression level （P＜0.05） in RCC.Sixty-six RCC patients were followed up for 6 to 67 months,patients with negative cytoplasm HuR or negative nucleus HIF1α expression had better overall survival rate and longer mean survival time than those with positive expression （P＜0.05）.Conclusions The expression levels of cytoplasm HuR and nucleus HIF-1α are associated with the clinical pathological parameter of RCC patients.HuR and HIF-1α may play an important role in the prognosis assessment of RCC patients.

关 键 词：癌 肾细胞 人抗原R 乏氧诱导因子1Α 诊断 预后 

分 类 号：R737.11[医药卫生—肿瘤]