HPLC测定草乌与瓜蒌配伍前后6种毒性成分量的变化  

Content changes of six toxic components before and after Aconiti Kusnezoffii Radix combined with Trichosanthis Fructus by HPLC

作　　者：张凌[1] 柳芳林 刘长安[1] 翟兴英[1] 廖清花 杨世林[1,2] 

机构地区：[1]江西中医药大学,江西南昌330004 [2]中药固体制剂制造技术国家工程研究中心,江西南昌330004

出　　处：《中草药》2014年第6期786-790,共5页Chinese Traditional and Herbal Drugs

基　　金：国家重点基础研究发展计划("973"计划)项目资助(2011CB505302)

摘　　要：目的研究草乌Aconiti Kusnezoffi Radix与瓜蒌Trichosanthis Fructus按照13个不同比例配伍后,苯甲酰新乌头碱、苯甲酰乌头原碱、苯甲酰次乌头原碱、中乌头碱、乌头碱、次乌头碱成分量的变化。方法草乌与瓜蒌的干燥粉末,混匀,分别用10倍量蒸馏水和75%乙醇浸润1 h,提取2次,每次1 h,合并提取液,离心,取上清液。HPLC采用Welch Xtimate C18(250 mm×4.6 mm,5μm)色谱柱,乙腈-40 mmol/L乙酸铵(氨水调pH 10.0)梯度洗脱;体积流量1 mL/min,检测波长235nm。结果草乌与瓜蒌配伍后,随瓜蒌比例增加,药液pH值逐渐降低,单酯型生物碱总量变化不明显,而双酯型生物碱总量增加。结论草乌与瓜蒌配伍后,从化学成分来看,双酯型生物碱类成分增加导致毒性增加。Objective To investigate the effect ofAconiti Kusnezoffii Radix （AKR） combined with Trichosanthis Fructus （TF） in 13 different ratios on contents of benzoylmesaconitine, benzoylaconine, benzoylhypaconine, mesaconitine, aconitine, and hypaconitine. Methods The powdered AKR-TF material, mixture, was alkalized by 10 times 75% ethanol and distilled water for 1 h, respectively, and extracted regurgitantly twice （each time for 1 h）. The extracts were combined and centrifuged, and the supernatant was taken. The Welch Xtimate-Cl8 colunm （250 mm× 4.6 mm, 5 μm） was eluted with a mobile phase consisted of aceto-nitrile and 40 mmol/L ammonium acetate （pH value 10.0, adjusted with aqueous ammonia） and gradient elution at a flow rate of 1 mL/min. The eluent was monitored by a UV detector at 235 nm. Results After AKR combined with TF, the content of monoester-alkaloids did not change significantly. Liquid pH value gradually reduced and total quality of diester-alkaloids increased with the ratios of TF increasing. Conclusion From the view of chemical composition, the toxicity is increasing when AKR combined with TF.

关 键 词：草乌 配伍 瓜蒌 毒性成分 HPLC 双酯型生物碱 单酯型生物碱 苯甲酰新乌头碱 苯甲酰乌头原碱 苯甲 酰次乌头原碱 中乌头碱 乌头碱 次乌头碱 

分 类 号：R286.02[医药卫生—中药学]