局部晚期宫颈癌组织中ERCC1 mRNA表达及DNA倍体与新辅助化疗效果的关系  

Relationship of expression of ERCC1 mRNA and DNA ploidy in locally advanced cervical cancer tissue to curative effect of neoadjuvant chemotherapy

作　　者：马一鸣[1,2] 陈红敏[1,2] 邓君丽[1,2] 阎夏 窦萌萌 王莉[1,2] 

机构地区：[1]郑州大学附属肿瘤医院妇瘤科 [2]河南省肿瘤医院妇瘤科,郑州450003

出　　处：《郑州大学学报（医学版）》2014年第2期233-236,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技攻关计划项目102102310107

摘　　要：目的：探讨局部晚期宫颈癌（ LACC）组织中ERCC1 mRNA表达和DNA倍体与新辅助化疗（ NACT）敏感性的关系。方法：收集60例LACC患者NACT前宫颈活检标本，应用PCR结合实时荧光探针技术分析标本中ER-CC1 mRNA的表达情况，采用细胞DNA定量分析技术分析DNA倍体。结果：60例患者中NACT有效33例，有效率为55．0％。 LACC组织中ERCC1 mRNA表达水平、DNA倍体与NACT疗效有关（ P＜0．05）；logistic回归模型分析结果也显示，LACC组织中ERCC1 mRNA高表达是NACT疗效的负性影响因素[β＝-2．672，OR（95％CI）＝0．069（0．008～0．583）]， DNA二倍体则是正性影响因素[β＝1．348，OR（95％CI）＝3．850（1．134～13．075）]。结论：LACC组织中ERCC1、DNA倍体表达与NACT敏感性密切相关。Aim：To explore relationship between the effect of neoadjuvant chemotherapy （ NACT） and the expression of ERCC1 mRNA,DNA ploidy in locally advanced cervical cancer （ LACC） tissue.Methods：A total of 60 cases of LACC biopsy specimens before chemotherapy were collected .PCR combined with the fluorescence probe technique were applied to analyze the expression of ERCC 1 mRNA in the specimens , and the DNA quantitative analysis technique was adopted to an-alyze DNA ploidy.Results：Thirty-three cases were effective in the 60 patients, and the effective rate was 55.0%.There was a correlation between ERCC1 mRNA expression, DNA ploidy and the curative effect of NACT（P〈0.05）.The result of logistic regression showed that high expression of ERCC 1 mRNA was a negative factor for curative effect of NACT [β=-2.672,OR（95%CI） =0.069 （0.008 -0.583）],and DNA diploid was a positive factor [β=1.348,OR（95%CI）=3.850（1.134-13.075）].Conclusion：The expression of ERCC1 mRNA and DNA ploidy in LACC tissue may be related to the sensitivity of NACT .

关 键 词：局部晚期宫颈癌 ERCC1 DNA倍体 新辅助化疗 

分 类 号：R737.33[医药卫生—肿瘤]