丝素纳米颗粒/β-环糊精菱形高分子微球的制备及结构与性能表征  

Preparation and Characterization of Silk Fibroin Nanoparticles/β-cyclodextrin Rhombus Polymer Microspheres

作　　者：刘小甜[1] 杨明英[1] 张海萍[1] 邓连霞[1] 朱良均[1] 

机构地区：[1]浙江大学应用生物资源研究所,杭州310058

出　　处：《蚕业科学》2014年第2期301-306,共6页ACTA SERICOLOGICA SINICA

基　　金：现代农业产业技术体系建设专项(No.CARS-22)

摘　　要：为了开发新型的药物缓释载体,以丝素纳米颗粒和β-环糊精为原料,以天然产物京尼平为交联剂,制备丝素纳米颗粒/β-环糊精菱形高分子微球。采用扫描电子显微镜(SEM)观察、红外光谱(FT-IR)分析、X射线衍射(XRD)分析、差示扫描量热(DSC)分析、热重(TG)分析等方法对制备的菱形高分子微球的结构和性能进行测试与表征。结果表明:制备的菱形高分子微球粒径在0.5~1.0μm之间,京尼平的添加使丝素纳米颗粒与β-环糊精发生融合,对菱形高分子微球的形成起到关键性作用;微球中丝素蛋白主要以β-折叠构象存在,丝素蛋白与京尼平交联后形成新的共价键,其结晶性能提高,热稳定性能增强。基于丝素纳米颗粒/β-环糊精菱形高分子微球的上述良好性能,以及原料组分自身的良好生物相容性和可降解性,预测其有望作为一种新型给药载体。To develop new sustained-release drug carriers, silk fibroin nanoparticles/β-cyclodextrin rhombus polymer mi- crospheres were prepared using silk fibroin nanoparticles and,8-cyclodextrin as raw material and the natural product ge- nipin as cross-linking agent. The structure and property of the rhombus polymer microspheres were investigated by scan- ning electronic microscopy （ SEM）, Fourier transform infrared （ FT-IR）, X-ray diffraction （ XRD）, differential scanning calorimetry （DSC） and thermogravimetry （TG）. The results indicated that the particle size of the prepared rhombus poly- mer microspheres was between 0.5 to 1.0 i^m. Addition of genipin could promote fusion of silk fibroin nanoparticles withβ-cyclodextrin, which played a key role in formation of the rhombus polymer microspheres. The predominant conformation of silk fibroin in the microspheres was,8-sheet, and new covalent bonds were formed after silk fibroin cross-linked with genipin. Both the crystallinity and thermal stability of silk fibroin were improved in rhombus polymer microspheres. Owing to their favorable properties, excellent biocompatiability and degradability of material components, the silk fibroin nanop- artictes/β-cyclodextrin rhombus polymer microspheres were considered to be a promising novel drug delivery carrier.

关 键 词：药物载体 丝素纳米颗粒 Β-环糊精 菱形高分子微球 结构 热稳定性 

分 类 号：TQ460.1[化学工程—制药化工] O631.3[理学—高分子化学]