亚急性二氯乙烷中毒性脑病大鼠模型的建立  

Establishment of a rat model of subacute toxic encephalopathy induced by 1, 2-dichloroethane

作　　者：邓燕君[1,4] 朱伟[2] 韦拔雄[3,4] 周旋[1,4] 苏艺伟 高原[1,4] 刘移民[3,4] 

机构地区：[1]中山大学公共卫生学院预防医学系广州,510080 [2]广州市疾病预防控制中心 [3]广州市职业病防治院 [4]广州市职业环境与健康效应实验室

出　　处：《中华劳动卫生职业病杂志》2014年第4期260-263,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：广州市“121人才梯队工程”后备人才培养项目;广东省“十二五”医学重点专科建设项目;广州市医学重点学科（2013-2015）建设项目

摘　　要：目的 建立亚急性1,2-二氯乙烷（DCE）中毒性脑病的动物模型.方法 60只SD大鼠随机分为阴性对照、阳性对照和DCE低剂量（1 472 mg/m3）、DCE中剂量（2 550 mg/m3）和DCE高剂量（4 418 mg/m3）组,每天静式吸入DCE6h,连续5d.阳性对照为腹腔注射脂多糖（5 mg/kg,8h后处死.染毒结束后采集血样并取脑组织,测定脑组织含水量并观察脑组织病理结构改变（HE染色）.结果 各DCE剂量组大鼠体重随DCE剂量升高而下降,差异均有统计学意义（P＜0.01）,脑组织含水量随DCE剂量升高而升高,中剂量组（80.09±0.14％）、高剂量组（80.28±0.10％）与阴性对照组（79.46±0.23％）相比有统计学差异（P＜0.001）.光学显微镜下显示DCE中剂量组的大鼠脑组织结构疏松、血管扩张,呈现明显脑水肿,高剂量组、阳性对照组大鼠脑组织呈现空泡状、海绵状,血管扩张严重,发生严重脑水肿.结论 亚急性1,2-二氯乙烷中毒性脑病大鼠模型成功建立.Objective To establish a rat model of 1,2-dichloroethane （DCE）-induced subacute toxic encephalopathy.Methods Sixty Sprague-Dawley rats were randomly divided into five groups：negative control,positive control,low-dose DCE （1 472 mg/m3）,middle-dose DCE （2 550 mg/m3）,and high-dose DCE （4 418 mg/m3）.The three DCE groups received static inhalation of DCE 6 hours a day for 6 consecutive days.The positive control group received intraperitoneal injection of lipopolysaccharide （5 mg/kg） and were sacrificed 8 hours after injection.Blood and brain tissue were collected,followed by determination of brain water content and HE staining for pathological examination of brain tissue.Results The rats in DCE groups suffered decreased body weight with increasing DCE dose （P〈0.01）,and brain water content rose with increasing DCE dose.The brain water content of middle-dose DCE group （80.09±0.14%） and high-dose DCE group （80.28±0.10%） increased significantly as compared with that of the negative control group （79.46±0.23%） （P〈0.001）.Optical microscopy discovered loose structure and vasodilation in the brain tissue of middle-dose DCE group,indicating obvious brain edema; the high-dose DCE group and positive control group had spongiform and vacuolated brain tissues with severe vascular dilation,indicating severe brain edema.Conclusion A rat model of subacute toxic encephalopathy induced by 1,2-dichloroethane has been successfully established.

关 键 词：亚急性 1 2-二氯乙烷 中毒性脑病 大鼠 

分 类 号：R-332[医药卫生]