瓜尔胶降解菌诱变及甘露聚糖酶特性研究  

Mutagenesis of the Guar Gum Degrading Strain and Characters of the Mannanase

作　　者：李慧玲[1,2] 刘祖艳[1] 赵敏[1] 

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]黑龙江中医药大学药学院,黑龙江哈尔滨150040

出　　处：《安徽农业科学》2013年第36期14041-14043,14046,共4页Journal of Anhui Agricultural Sciences

摘　　要：为了筛选降解瓜尔胶的菌株,从亚麻液中筛选到1株降解瓜尔胶产β-甘露聚糖酶的细菌HL-28,经形态观察、生理生化特性和16S rDNA序列分析鉴定其为金黄杆菌(Chryseobacterium)。菌株HL-28产甘露聚糖酶的活力达64.532 U/ml,经紫外诱变育种获得突变株HL-28-30,酶活提高到339.787 U/ml,提高了426.54%。粗酶发挥催化作用的最适pH值为5.6,最适反应温度50℃。In order to screen the strains degraded guar gum,a β-mannanase-producing strain HL-28,was isolated from flax liquid in this study.The stain HL-28 was identified as Chryseobacterium according to the results of physiological and biochemical tests as well as 16S rDNA sequence analysis.The activity of mannanase by strain HL-28 achieved 64.532 U/ml.After the strain HL-28 was treated with ultraviolet radiation,a mutant strain HL-28-30 was obtained and its activity reached to 339.787 U/ml,which increased 426.54％.The optimal pH value of crude enzymes is 5.6 and its optimum reaction temperature is 50 ℃.

关 键 词：瓜尔胶 紫外诱变 酶学性质 

分 类 号：S188[农业科学—农业基础科学]