肢体缺血后处理促进缺血性脑损伤大鼠内源性神经干细胞的增殖  

Limb ischemic postconditioning promotes the proliferation of endogenous neural stem cells in rats with cerebral ischemia injury

作　　者：杜郭佳[1,2] 党木仁[2] 谭泽明[1] 苏日青[2] 沈潜[3] 方加胜[1] 

机构地区：[1]中南大学湘雅医院神经外科,湖南省长沙市410008 [2]新疆医科大学第一附属医院神经外科,新疆维吾尔自治区乌鲁木齐市830054 [3]新疆医科大学第四附属医院神经外科,新疆维吾尔自治区乌鲁木齐市834000

出　　处：《中国组织工程研究》2014年第10期1597-1602,共6页Chinese Journal of Tissue Engineering Research

基　　金：新疆维吾尔自治区青年科学基金项目(2011211B31)~~

摘　　要：背景:远程肢体缺血预处理应用到脑缺血-再灌注损伤领域的研究已有一些报道,但肢体缺血后处理应用于该领域报道很少。目的:观察肢体缺血后处理对缺血性脑损伤大鼠侧脑室旁神经干细胞增殖的影响。方法:利用改良线栓法制作局灶性脑缺血大鼠模型,将30只造模成功的SD大鼠随机分为实验组和对照组,每组15只。实验组行远程肢体缺血后处理,对照组不做处理。2组大鼠分别于造模后5,10,15 d处死取脑,处死前1 d每隔8 h腹腔注射50 mg/kg 5-嗅脱氧尿嘧啶核苷1次,共3次。应用免疫组织化学方法检测梗死灶区大鼠脑组织5-嗅脱氧尿嘧啶核苷阳性细胞数。结果与结论:与对照组比较,实验组脑再灌注损伤程度呈现不同程度减轻,行为学评分降低(P<0.05)。实验组各时间点梗死灶周边皮质的5-嗅脱氧尿嘧啶核苷阳性细胞数明显多于对照组(P<0.05)。提示局灶性脑缺血造模后的肢体缺血后处理可以改善大鼠行为学表现,促进了内源性干细胞的激活增殖,对缺血性脑损伤有保护作用。BACKGROUND：There are a few reports addressing application of limb ischemic preconditioning in cerebral ischemia-reperfusion injury, but few reports have addressed limb ischemic postconditioning applied in this field. OBJECTIVE：To explore the effects of limb ischemic postconditioning on the proliferation of neural stem cells of rats after cerebral ischemia. METHODS：Focal stroke models were established in rats, and then 30 rat models were randomly divided into experimental group （n=15） and control group （n=15）. Remote limb ischemic postconditioning was applied in the experimental group, but not in the control group. 5-Bromo-2-deoxyuridine （BrdU;50 mg/kg） was injected intraperitoneal y into rat models three times a day, and then the rat brains were respectively obtained at days 5, 10 and 15 days in two groups. The amount of BrdU-positive cells was measured by immunohistochemistry method in two groups.

关 键 词：干细胞 神经干细胞 肢体缺血后处理 缺血性脑损伤 细胞增殖 5-嗅脱氧尿嘧啶核苷 

分 类 号：R394.2[医药卫生—医学遗传学]