家蚕两种新的类鹑斑突变体的遗传分析和SSR标记定位  

Genetic analysis and gene mapping of two novel quail-like mutants from the silkworm(Bombyx mori)

作　　者：赵巧玲[1,2] 王文波[1] 陈安利[1] 裘智勇[1,2] 夏定国[1,2] 钱荷英[1,2] 沈兴家[1,2] 

机构地区：[1]江苏科技大学蚕业研究所,镇江212003 [2]中国农业科学院蚕业研究所,农业部蚕桑遗传改良重点开放实验室,镇江212018

出　　处：《遗传》2014年第4期369-375,共7页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:31372378);江苏省科技支撑计划(农业)项目(编号:BE2011327-2);"十二五"国家支撑计划项目(编号:2011BAD33B04)资助

摘　　要：在家蚕品种选育过程中发现了两种斑纹突变体,与普通斑相比,其幼虫眼状纹不明显,而半月纹和星状纹正常,其间有点和线构成鹑状斑纹,第6、7腹节背面布有纵向波纹状斑纹,整体斑纹与鹑斑(quail,q)极其相似,暂且命名为类鹑斑(quail-like,q-l)。其中一种突变体稚蚕期体色呈褐色,蚕体发育正常,蚕茧大小一致,茧型正常,称为褐色类鹑斑(brown quail-like,q-lb);另一种突变体幼虫体色为浅粉紫色,幼虫食桑量少,发育缓慢,体质较弱,体型较小,茧型偏小,称为紫色类鹑斑(purple quail-like,q-lp)。遗传分析表明,两个类鹑斑基因均为隐性基因;褐色类鹑斑(q-lb)与紫色类鹑斑(q-lp)为等位基因,紫色类鹑斑(q-lp)对褐色类鹑斑(q-lb)为隐性。经与形态标记P3(2)、p(2)、Ze(3)、L(4)、re(5)、E(6)、q(7)、I-a(9)、ms(12)、ch(13)、oa(14)、cts(16)、mln(18)、msn(19)、rb(21)、so(26)测验和SSR分子标记多态性分析,新发现的两种类鹑斑不同于鹑斑(q),其基因座位于第8连锁群。Two novel body marking mutants were discovered during silkworm （Bombyx mori） breeding.The mutants have no obvious eye-spots compared with normal marking （＋） individuals,but their star spots and semilunar markings on dorsal sides are normal,and there are dots and lines with longitudinal wave markings on dorsal sides of the 6th to 7th abdominal segments which consist quail markings in between star spots and semilunar markings.The whole body markings are very similar to that of quail mutant （q）; thus these mutants are named as quail-like mutants （q-l）.Young larvae of one mutant are in brown color,and develop normally.Their cocoons are regular and uniform in size.Thus,this mutant is designated as brown quail-like （q-lb）.Another mutant＇s larvae are in light purple skin; thus this mutant is named as purple quail-like （q-lp）.They take little amount of mulberry leaves,and are weak and develop slowly and unevenly.Their larval bodies and cocoons are small.Genetic analysis revealed that both q-lb and q-lp were recessive genes,and they were allelic,with q-lb recessive to q-lp.These genes are different from quail mutant （q） and located on the chromosome 8 after tested by the morphological markers,p3（2）,p（2）,Ze（3）,L（4）,re（5）,E（6）,q（7）,I-a（9）,ms（12）,ch（13）,oa（14）,cts（16）,mln（18）,msn（19）,rb（21） and so（26） and SSR markers.

关 键 词：家蚕 突变体 类鹑斑 等位基因 SSR分子标记 

分 类 号：S881.2[农业科学—特种经济动物饲养]