掺锶仿生涂层用于促进种植体早期骨结合的初步研究  

Effects of strontium-substituted biomimetic implant surfaces on early osseointegration

作　　者：高麒 牛强[2] 李永锋 沈舒宁 张永强 申明明 孔亮 李永明 

机构地区：[1]第四军医大学口腔医院正畸科军事口腔医学国家重点实验室,陕西西安710032 [2]河北医科大学第二医院口腔颌面外科,河北石家庄050000 [3]第四军医大学口腔医院颌面外科军事口腔医学国家重点实验室,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2014年第4期216-220,共5页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(30900284)

摘　　要：目的:在种植体表面构建掺锶的仿生化涂层并观察其对早期骨结合的影响。方法:采用酸蚀加磁控溅射技术在纯钛种植体表面产生掺锶的微纳米化仿生涂层(实验组);用扫描电镜、能量色散型X射线谱(EDS)观察其表面形貌和元素成分,将实验组种植体植入SD大鼠一侧股骨干骺端,另一侧植入机械加工的种植体作为对照。术后进行双荧光序列标记,饲养4周时取材,用丽春红染色、荧光染色分析评估2组种植体早期骨结合的能力,轴向拔出实验评估其生物力学性能。结果:实验组种植体表面形成了微纳米化的仿生涂层,其表面锶的含量1.2%,提示成功构建掺锶的微纳米化种植体涂层;实验组新生骨组织与种植体结合紧密,成骨活跃,骨接触率(BIC)以及骨矿化沉积率(MAR)均显著高于对照组(P<0.05);实验组的最大拔出力明显高于对照组(P<0.05)。结论:掺锶的微纳米化仿生涂层可有效促进种植体的早期骨结合能力。AIM： To examine the coating of strontium-substituted titanium implant and to observe the effects of the coating surface on early osseointegration in vivo. METHODS ： Strontium-substituted biomimetic surface titanium implants were manufactured by radio-frequency magnetron sputtering. The morphology and the component of the coating were examined by SEM and energy dispersive X-ray spectroscopy （EDS）. The surface-coated implants （ experimental group） and machanically-surfaced implants （control group） were randomly inserted into the distal femura of 20 SD rats, dual fluorochrome sequential labeling was used for osseointergration observation. 4 weeks after the surgery, the morphology was observed by histological examination. The maximal pull-out force of the specimens was measured by universal material testing machine. RESULTS： The strontium-substituted coating presented a micro/nanorough structure, in the coating the content of Sr was 1.2%. Stronger osteogenic activity at early stage of bone healing was observed in experimental group. The bone implant contact, mineral apposition ration and maximal pull-out forces of experimental group were significantly higher than those of control group （P 〈 0. 05 ）. CONCLUSION： Modification with strontium-substituted biomimetic surface can promote osseointegration of dental implants in early stage of implantation.

关 键 词：种植体 早期骨结合 锶 表面处理 

分 类 号：R783.1[医药卫生—口腔医学]