膨体聚四氟乙烯与人脂肪干细胞的体外生物相容性  

In vitro biocompatibility of expanded polytetrafluoroethylene scaffold and human adipose-derived stem cells

作　　者：杨柳[1] 姜南[1] 徐扬阳[1] 朱梦琳[1] 曹菁[1] 

机构地区：[1]郑州大学第五附属医院整形美容科,河南省郑州市450052

出　　处：《中国组织工程研究》2014年第12期1932-1937,共6页Chinese Journal of Tissue Engineering Research

基　　金：河南省科技厅项目(041000531010)~~

摘　　要：背景:膨体聚四氟乙烯多孔高分子聚合材料是临床常用的植入假体,具有良好的生物相容性,不易变形、变质,不产生炎症吸收反应,可允许细胞游走和组织向内生长。目的:观察人脂肪干细胞与膨体聚四氟乙烯材料的生物相容性。方法:将第4代人脂肪干细胞与膨体聚四氟乙烯体外复合培养,采用倒置相差显微镜观察细胞在支架上黏附、生长及增殖情况,计算细胞黏附率,MTT比色法检测细胞增殖率。结果与结论:刚接种的细胞呈圆形透亮,在支架材料表面分布均匀,细胞活性佳,3 h后大量细胞贴壁,24 h后可见少量呈短梭形的脂肪干细胞贴壁,3 d首次换液,细胞清晰可见,低密度生长时呈短梭形或多角形,分布均匀,种植7 d后细胞数量明显增加,极少细胞从支架上掉落,细胞黏附率平均达95.7%,并且细胞仍保持正常的分裂增殖速度。说明膨体聚四氟乙烯材料具有良好的细胞相容性。BACKGROUND：Expanded polytetrafluoroethylene is a kind of porous polymer materials which is commonly used as clinical implants, and it has good biocompatibility, and is not easy to deformation or metamorphism. There is no existence of inflammation absorption reaction, and it al ows the cel migration and tissue ingrowth. OBJECTIVE：To study the biocompatibility of expanded polytetrafluoroethylene scaffold and human adipose-derived stem cel s. METHODS：The passage 4 human adipose-derived stem cel s were co-cultured with expanded polytetrafluoroethylene scaffold in vitro. The morphology and function of cel s adhered to the scaffold were observed by inverted phase contrast microscope, and cel adhesive rates and proliferation rates were also calculated by MTT assay. RESULTS AND CONCLUSION：The inoculated cel s were round and bright, distributed on the surface of scaffolds uniformly, with good cel viability. After 3 hours a large number of adherent cel s were observed from the micrograph;after 24 hours there were a smal amount of short-spindle adipose-derived stem cel s. After cultured for 3 days, the short fusiform or polygon cel s could be seen clearly. After cultured for 7 days, the number of cel s increased significantly, few cel s fel off from the scaffold, and cel adhesion rate was up to an average of 95.7%. Meanwhile, the cel s revealed normal splitting proliferation rate. These findings indicate that human adipose-derived stem cel s are able to attach, grow and proliferate wel on the scaffold. Expanded polytetrafluoroethylene reveals excel ent cel ular compatibility and can be used as a vehicle for adipose tissue engineering.

关 键 词：生物材料 材料相容性 聚四氟乙烯 人脂肪干细胞 支架 组织工程 生物相容性 

分 类 号：R394.2[医药卫生—医学遗传学]