p53和自噬相关基因Beclinl在唾液腺多形性腺瘤和癌在多形性腺瘤中的表达及意义  

Expression and clinical significance of p^(53) and autophagy-related gene Beclinl in salivary pleomorphic adenoma and carcinoma in pleomorphic adenoma

作　　者：陈国昆 吴益华[3] 张世周[1,3] 张东升[1,3] 黄圣运[3] 温洁[1,2] 

机构地区：[1]山东大学口腔医学院,山东济南250012 [2]山东省口腔生物医学重点实验室,山东济南250012 [3]山东大学附属省立医院口腔科,山东济南250021

出　　处：《上海口腔医学》2014年第2期214-218,共5页Shanghai Journal of Stomatology

摘　　要：目的:探讨p53、自噬相关基因Beclin1的表达及与唾液腺多形性腺瘤之间的关系。方法:应用免疫组化方法检测108例唾液腺多形性腺瘤、20例癌在多形性腺瘤中的p53及自噬相关基因Beclin1的表达,并结合临床病理因素进行分析。采用SPSS20.0软件包对数据进行统计分析。结果:p53在多形性腺瘤中的阳性率(9%)显著低于癌在多形性腺瘤中(14%)(P<0.001);Beclin1在多形性腺瘤中的阳性率(91%)则显著高于癌在多形性腺瘤中(11%)(P<0.001),但2种基因的表达与患者的性别、年龄、生长时间、肿瘤大小、位置和复发等(P>0.05)无显著相关性。p53与Beclin1在多形性腺瘤中的表达呈负相关(r=-0.330,P<0.05)。结论:p53与Beclin1蛋白表达水平与多形性腺瘤发生、发展关系密切。肿瘤发生过程中,细胞自噬作用降低和抗凋亡作用增强同时并存,提高自噬能力将成为肿瘤治疗的又一途径。PURPOSE： To investigate the expression of p53 and Beclinl in salivary pleomorphic adenoma （PA） and its clinical significance. METHODS： The expression of p53 and Beclinl were assessed by immunohistochemistry in 108 cases of PA and 20 cases of carcinoma in pleomorphic adenoma （CIPA）. The results were used to analyze the relationship between gene expressions and the development of PA as well as the clinical pathological features. Statistic analysis was conducted with SPSS 20.0 software package. RESULTS： The positive expressions of p53 in PA samples （9%） were significantly lower than that in CIPA （14%） （P〈0.001）. The positive expressions of autophagy-related gene Beclinl in PA samples （91%） were significantly higher than that CIPA （11%） （P〈0.001）. The expression levels of these genes were not associated with gender, age, clinical course, tumor size, and location of PA （P〉0.05）. There was a negative correlation between p53 and Beclinl expression in PA （r---0.330,P〈0.05）. CONCLUSIONS： The expression levels of Beclinl and p53 protein are closely related to the development of PA. Reduced autophagy and enhanced anti apoptosis coexist in the process of tumor formation. Thus, raising the autophagy ability may become another alternative choice for cancer therapy,

关 键 词：自噬 P53 BECLIN1 多形性腺瘤 

分 类 号：R739.87[医药卫生—肿瘤]