转录因子CDX2过表达对人胃癌SGC-7901细胞增殖和细胞周期的影响  

Effect of over-expression of transcription factor CDX2 on proliferation and cell cycle of human gastric cancer cell line SGC-7901

作　　者：曹稳珑 韦尉元[1] 张笑石 罗文[1] 严林海[1] 谢玉波[2] 肖强[1] 

机构地区：[1]广西医科大学第一附属医院胃肠腺体外科,广西南宁530021 [2]广西医科大学第一附属医院麻醉科,广西南宁530021

出　　处：《中国病理生理杂志》2014年第4期620-624,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81060201);广西科学技术攻关项目(No.1298003-2-6);广西自然科学基金资助项目(No.2011GXNSFA018273)

摘　　要：目的:探索CDX2过表达对人胃癌SGC-7901细胞增殖、生长和细胞周期的影响及其分子机制。方法:采用携带CDX2基因的重组慢病毒颗粒(LV-CDX2-GFP)感染SGC-7901细胞,作为实验组(LV-CDX2-GFP组);以对照慢病毒颗粒(LV-GFP)感染SGC-7901细胞,作为阴性对照组(LV-GFP组);空白对照组常规培养,不做任何处理。分别采用CCK-8法检测细胞的增殖活力,流式细胞术检测各组细胞周期的分布,半定量逆转录-聚合酶链反应(RT-PCR)和Western blotting技术检测细胞中CDX2、Bax、Bcl-2、cyclin D1和survivin mRNA和蛋白的表达。结果:与LV-GFP组和空白对照组比较,LV-CDX2-GFP组细胞增殖活力明显降低(P<0.05),G0/G1期所占比例上升(P<0.05),Bcl-2、cyclin D1和survivin mRNA和蛋白的表达降低(P<0.05),Bax mRNA和蛋白的表达上调(P<0.05),而LV-GFP组与空白对照组比较,差异无统计学意义(P>0.05)。结论:慢病毒介导的CDX2过表达抑制胃癌细胞增殖和生长,使细胞周期停滞在G0/G1期,其机制可能与CDX2过表达使胃癌细胞Bcl-2、cyclin D1、survivin表达下调和Bax表达上调有关。AIM： To study the effect and the molecular mechanism of CDX2 over-expression on the prolifera- tion, growth and cell cycle of human gastric cancer cell line SGC-7901. METHODS： The SGC-7901 cells in LV-CDX2- GFP group were transfected with the recombinant lentivirus vector LV-CDX2-GFP, the cells in LV-GFP group were trans- fected with the negative control lentiviral vector for the negative control, and the cells in blank control group were without any treatment. The cell proliferation was detected by CCK-8 assay. The cell cycle distribution was analyzed by flow cytome- try. The expression of CDX2, Bax, Bcl-2, cyclin D1 and survivin was determined by semi-quantitative RT-PCR and Wes- tern blotting. RESULTS： Compared with LV-GFP group and blank control group, the proliferation activity of the SGC- 7901 cells was significantly lower （P 〈 0. 05 ）, the G0/G~ phase proportion increased （P 〈 0. 05 ）, the mRNA and protein levels of Bcl-2, cyclin D1 and survivin were reduced （P 〈 0. 05 ）, and the mRNA and protein levels of Bax were up-regula- ted （P 〈 0. 05） in LV-CDX2-GFP group. No statistically significant difference of the above indexes was observed （ P 〉 0. 05 ） between LV-GFP group and blank control group. CONCLUSION： Over-expression of CDX2 mediated by lentivirus inhibits the proliferation and growth of human gastric cancer SGC-7901 ceils and arrestes the cell cycle at G0/G1 phase, which may be related to down-regulation of Bcl-2, cyclin D1 and survivin and up-regulation of Bax.

关 键 词：胃肿瘤 SGC-7901细胞 转录因子CDX2 

分 类 号：R735.2[医药卫生—肿瘤]