负向调控miR-9抑制鼻咽癌细胞的增殖、迁移和侵袭  

Inhibition of miR-9 expression suppresses proliferation,invasion and migration of nasopharyngeal carcinoma cells

作　　者：郑朝攀[1] 韩灵[1] 侯伟坚[1] 文译辉[1] 傅然[1] 文卫平[1] 

机构地区：[1]中山大学附属第一医院耳鼻咽喉科医院,中山大学耳鼻咽喉科学研究所,广东广州510080

出　　处：《中国病理生理杂志》2014年第4期640-644,共5页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目(重点项目)(No.10251008901000023)

摘　　要：目的:探讨负向调控miR-9对人鼻咽癌细胞增殖、迁移和侵袭作用。方法:用脂质体LipofectamineTM2000转染合成抑制剂的方法抑制鼻咽癌细胞miR-9表达,转染抑制对照剂作为对照组。CCK-8法和流式细胞术检测细胞增殖和细胞周期变化;Transwell侵袭实验和划痕实验检测细胞侵袭和迁移能力;免疫印迹实验检测蛋白变化。结果:抑制鼻咽癌细胞miR-9表达后,肿瘤增殖能力降低(P<0.05),G0/G1期细胞增多[CNE2:(57.96±1.39)%vs(47.93±1.76)%,P<0.05;CNE1:(51.24±0.88)%vs(48.29±0.39)%,P<0.05],迁移距离明显缩短[CNE2:(186.50±7.94)μm vs(247.56±15.56)μm,P<0.05;CNE1:(139.06±16.73)μm vs(230.66±14.27)μm,P<0.01],CNE2细胞中侵袭细胞数明显减少(43.00±3.17 vs 65.80±5.20,P<0.01),β-连环蛋白(β-catenin)表达被抑制。结论:在鼻咽癌细胞中,负向调控miR-9可抑制鼻咽癌细胞的增殖、侵袭和迁移。AIM： To investigate the effects of down-regulated miR-9 expression on the proliferation, invasion and migration of nasopharyngeal carcinoma （NPC） cells. METHODS： Human NPC CNE1 and CNE2 ceils were transfect- ed with the inhibitor of miR-9 by Lipofectamine to down-regulate the expression of miR-9, and the cells transfected with an inhibitor control were also set up. The cell proliferation and cell cycle were evaluated by CCK-8 assay and flow cytometry. The cell invasion and migration abilities were detected by Transwell invasion and wound-healing assays. Immunoblotting was applied to analyze the levels of the proteins. RESULTS： Compared with control group, inhibition of miR-9 expression in the NPC cells by transfection of the miR-9 inhibitor significantly decreased the proliferation ability （ P 〈 0. 05 ）. The per- centages of the ceils in G0/G1 phase [ CNE2 ： （57.96 ＋ 1.39 ） % vs （47. 93 ± 1.76 ） %, P 〈 0. 05 ; CNE1 ： （ 51.24± 0. 88） % vs （48.29 ＋ 0. 39 ） %, P 〈 0. 05 ] were significantly increased. The migration distances [ CNE2 ： （ 186. 50 ± 7.94） Ixm vs （247.56 ＋ 15.56） um, P 〈0. 05 ; CNE1 ： （ 139.06 ± 16. 73） um vs （230. 66 ± 14. 27） um, P 〈0. 01 ] and the invasion ability of the CNE2 ceils （43. 00 ± 3.17 vs 65.80 ± 5.20, P 〈 0. 01 ） were also significantly inhibited. Moreo- ver, the tumor cells transfected with the inhibitors produced lower B-catenin. CONCLUSION： Inhibition of miR-9 expres- sion suppresses the proliferation, invasion and migration of nasopharyngeal carcinoma cells.

关 键 词：鼻咽肿瘤 MIR-9 细胞增殖 肿瘤侵袭 

分 类 号：R363[医药卫生—病理学]