非洲马瘟VP7蛋白多克隆抗体的制备及IgM捕获ELISA检测方法的建立  

Preparation of Polyclonal Antibody Against Protein VP7 of African Horse Sickness Virus and Development of IgM capture Enzyme-linked Immunosorbent Assay for Detecting African Horse Sickness

作　　者：郑小龙[1] 朱来华[1] 王群[1] 艾军[2] 邓明俊[1] 肖西志[1] 梁成珠[1] 姜帆[1] 于业锋[1] 

机构地区：[1]山东出入境检验检疫局,山东青岛266002 [2]云南出入境检验检疫局,云南昆明650000

出　　处：《中国动物检疫》2014年第5期70-73,共4页China Animal Health Inspection

摘　　要：为建立早期快速检测非洲马瘟抗体的IgM捕获ELISA(MAC-ELISA)方法,本试验利用大肠杆菌表达出具有良好抗原性的VP7蛋白,并制备该蛋白的多克隆抗体,通过优化抗原浓度、血清稀释度及酶标抗体稀释度等,建立了检测非洲马瘟的MAC-ELISA方法,并进行了初步应用。结果表明,利用重组表达的VP7蛋白成功建立了检测非洲马瘟的MAC-ELISA方法。The study was aimed to develop an IgM capture enzyme-linked immunosorbent assay for detecting African horse sickness （AHS） . The AHSV VP7 protein with good immunogenicity was expressed in BL21 and polyclonal an- tibody against protein VP7 of African horse sickness virus was prepared. An IgM capture enzyme-linked immunosorbent assay for detecting AHS was developed by optimizing the antigen concentration, the second antibody concentration and the serum dilution. Preliminary application of the MAC-ELISA method indicated that the MAC-ELISA with the recom- bined VP7 protein was successfully developed for detecting antibodies to AHSV with good sensitivity and specificity.

关 键 词：非洲马瘟 IgM捕获ELISA(MAC—ELISA) VP7蛋白 

分 类 号：S852.652[农业科学—基础兽医学] S855.3[农业科学—兽医学]