药物肾毒性生物标志物检测方法研究进展  

Research Progress About Detection Methods of Drug-Induced Nephrotoxicity Biomarkers

作　　者：朱聪[1,2] 黄芝瑛[2] 周晓冰[1] 李波[1] 

机构地区：[1]中国食品药品检定研究院国家药物安全评价监测中心,药物非临床安全评价研究北京市重点实验室,北京100176 [2]中山大学药学院药物安全性评价中心,广州510006

出　　处：《中国药学杂志》2014年第9期707-711,共5页Chinese Pharmaceutical Journal

基　　金：科技部"十二五"关键技术研究课题--毒性生物标志物关键技术研究(2012ZX09505001-004);中国食品药品检定研究院中青年发展研究基金(2011C3)

摘　　要：目的对近年来肾毒性生物标志物检测方法现状和研究进展作一综述。方法查阅国内外肾毒性生物标志物的临床前和临床研究文献和相关检测方法的论著,对各检测方法及其实际应用进行描述和总结。结果与结论多重检测方法如Luminex xMAP等运用越来越多,其比酶联免疫吸附测定和蛋白质免疫印迹技术更快速高效,但是多重检测方法仍需要在实际应用中进行方法间的比较和验证。另外,不依赖于抗原抗体反应的检测方法和基于毒理基因组学的方法正在发展。这些新的检测方法将大大推进药物肾毒性生物标志物的发现和验证。OBJECTIVE To review the research status and progress about detection methods of drug-induced nephrotoxicity bio markers in recent years. METHODS By looking up papers and books of preclinical and clinical research on nephrotoxieity biomark ers and related detection methods, we made description and summary on different detection methods and their practical application. RESULTS AND CONCLUSION Multiplex assays such as Luminex xMAP have been used more frequently, they are faster and more efficient than ELISA and Western blot. However, multiplex assays still need to be compared and verified in the practical use. In addi tion, methods which are independent of antigen-antibody reaction and which are based on toxicogenomics have been developed. These new detection methods will greatly improve the discovery and confirmation of new nephrotoxicity biomarkers.

关 键 词：药物肾毒性 生物标志物 检测方法 

分 类 号：R944[医药卫生—药剂学]