粘红酵母和地衣芽孢杆菌菌肥对芸豆豆苗生长的影响  

Effects of Bio-fertilizer of Rhodotorula glutinis and Bacillus licheniformis on Kidney Bean Growth

作　　者：薛飞燕[1,2] 赵爽[1] 玉晏飞[1] 马兰青[1,2] 赵晓萌[1] 

机构地区：[1]北京农学院生物科学与工程学院,北京102206 [2]农业部都市农业(北方)重点实验室,北京102206

出　　处：《安徽农业科学》2014年第12期3541-3543,3634,共4页Journal of Anhui Agricultural Sciences

基　　金：北京市教委面上基金项目(KM201210020009);北京市优秀人才基金项目(2013D005021000003)

摘　　要：[目的]为了探索餐厨废水资源化利用,以生产生物肥料的可行性。[方法]以餐厨废水为原料,分别接种粘红酵母(R.glutinis)和地衣芽孢杆菌(B.licheniformis)进行发酵培养,获得生物菌肥,以基肥形式进行芸豆盆栽肥效测试,分别测定了粘红酵母菌肥、地衣芽孢杆菌菌肥及二者的混合菌肥对芸豆豆苗生长的影响。[结果]粘红酵母利用餐厨废水发酵24 h以后可积累生物量10 g/L以上,地衣芽孢杆菌利用餐厨废水发酵60 h后高温淀粉酶活力可达950 U以上;施以单一菌肥时,粘红酵母菌肥和地衣芽孢杆菌菌肥对豆苗径粗增长均有促进作用(前者的促进作用更明显),而对豆苗株高增长有明显的抑制效应;以不同剂量、不同比例施以混合菌肥时,当粘红酵母与地衣芽孢杆菌质量比为4∶1,总施肥量为0.2 g/盆时,混合菌肥对径粗的促进作用最大,对株高的抑制效应最小。[结论]粘红酵母和地衣芽孢杆菌的混合菌对芸豆豆苗径粗增长有明显的促进作用,可作为生物肥料使用。[ Objective ] The objective of this study was to test the feasibility of converting restaurant wastewater into bio-fertilizer. [ Method ] Restaurant wastewater was used as raw material for Rhodotorula glutinis and Bacillus licheniformis fermentation. As basal bio-fertilizer, the effects ofR. glutinis and B. licheniformis on kidney bean growth was tested. [Resuh] More than 10 g/L ofR. glutinis biomass and 950 U of amylase could be obtained respectivelY. In the pot experiment, both R. glutinis and B. licheniformis promoted the diameter and inhibited the height of kidney bean plant during the cultivation. Different effects were found with different doses and different proportions of the mixed bio- fertilizer. Maximum promotion effect on plant diameter and minimum inhibitory effect on plant height was observed when the ratio of R. glutinis and B. licheniformis was 4：1 and the dose of mixed bio-fertilizer was 0.2 g in every pot. [ Conclusion] The mixed bio-fertilizer of R. glutinis and B. licheniformis promoted diameter growth of kidney bean, which could be used as bio-fertilizer.

关 键 词：粘红酵母 地衣芽孢杆菌 菌肥 芸豆 

分 类 号：S144[农业科学—肥料学]