KiSS-1基因对人胰腺癌Capan-2细胞的增殖及侵袭的影响  

Effects of KiSS-1 on Human Pancreatic Carcinoma Capan-2 Cell Proliferation and Invasion

作　　者：王春晖[1] 乔宠[2] 周文平[1] 麻树仁[3] 

机构地区：[1]中国人民解放军沈阳军区总医院肝胆外科,沈阳110016 [2]中国医科大学附属盛京医院妇产科,沈阳110004 [3]中国人民解放军沈阳军区总医院内镜中心,沈阳110016

出　　处：《中国医科大学学报》2014年第4期304-307,312,共5页Journal of China Medical University

基　　金：全军医学科学技术研究"十一五"计划青年学者课题(06Q014);辽宁省博士启动基金(20071038)

摘　　要：目的研究KiSS-1基因对人胰腺癌Capan-2细胞增殖、侵袭的影响。方法经脂质体介导瞬时转染上调Capan-2细胞中KiSS-1基因的表达,采用实时荧光定量PCR和Western blot技术检测Capan-2细胞中KiSS-1 mRNA及其蛋白肽metastin的表达。采用MTT法分析KiSS-1对Capan-2细胞增殖能力的影响;Matrigel铺被的transwell侵袭实验检测KiSS-1对Capan-2细胞侵袭能力的作用。结果 KiSS-1转染Capan-2细胞36h KiSS-1 mRNA开始增多,到48 h KiSS-1 mRNA及其蛋白肽metastin表达明显增强,达到峰值。与空质粒转染组和空白对照组相比,KiSS-1转染Capan-2细胞48 h后,细胞体外侵袭能力显著下降,侵袭指数显著降低(P均<0.01),侵袭力抑制率达到56.5%(P均<0.01),但转染KiSS-1对Capan-2细胞增殖能力无明显影响(P均>0.05)。结论KiSS-1是胰腺癌的转移抑制基因,可作为基因治疗的新靶点。Objective To investigate the effects of KISS- 1 on the proliferation and invasion abilities of human pancreatic carcinoma Capan-2 cells. Metods Transient lransfecfion with Lipofectamine was used to up-regulate the expression of KISS- 1 in Capan- 2 cells. Tested the expression of KISS-1 mRNA and Kisspeptin-metastin by real-time quantitative PCR and Western blot. MTF assay was used to detect the influence of KISS-1 on Capan-2 cell proliferation. Transwell assay with Matrigel coated was used to evaluate the in vitro invasion ability. Results The KISS- 1 mRNA ex- pression of Capan-2 cells was significantly up-regulated 36 hours after transfection. KISS-1 mRNA and metasfin reached the peak 48 hours after transfection. Compared with empty plasmid transfected group and blank control group, invasion ability and invasion index of Capan-2 cell was signifi- cantly decrease 48 hours after K/SS- 1 transfection （ P 〈 0.01 ）, meanwhile, the inhibition rates of Capan-2 cell invasiveness was 56.5%.There were no significant difference of proliferation after transfection compared with empty plasmid group and blank control group （P 〈 0.05 ）. Conclusion KiSS-1 is a metastasis suppressor gene of pancreatic cancer, which can be used as a novel target for gene therapy.

关 键 词：KISS-1 胰腺癌 增殖 侵袭 

分 类 号：R735.9[医药卫生—肿瘤]