抗人乳腺癌单链抗体重组免疫毒素的构建及初步表达  

Construction and Expression of a Recombinant Immunotoxin of Single-chain Antibody Against Human Breast Cancer

作　　者：彭茜[1] 高杨[1] 王靖[2] 田帜江 王德发[1] 张霖[2] 

机构地区：[1]南开大学医学院,天津300071 [2]天津医科大学附属肿瘤医院中心实验室,天津300060 [3]国药控股新疆新特西部药业有限公司,新疆乌鲁木齐830054

出　　处：《南开大学学报（自然科学版）》2013年第6期64-69,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：天津市自然科学基金(013609111);国家自然科学基金(81101567)

摘　　要：通过基因克隆技术从绿脓杆菌基因组中扩增出其外毒素基因PE40.利用基因重组技术,将PE40与已克隆的抗人乳腺癌单链抗体基因M4G3/ScFv[1]相互连接,构建出完整的抗人乳腺癌重组免疫毒素M4G3/ScFv-PE40融合基因,导入表达载体PET21a进行初步表达,得到重组蛋白M4G3/ScFv-PE40,体外细胞亲和性实验证实其可特异性识别并结合T47D人乳腺癌细胞,为进一步的融合蛋白高效表达及细胞毒实验打下基础,可望为乳腺癌的临床治疗提供一种新的靶向药物.The PE40fragment was amplified by PCR from genome of pseudomonas exotoxin A and connected with cloned single-chain antibody against human breast cancer gene which named M4G3/ScFv.The fusion gene was cloned into an expression vector PET21.After induced by IPTG,the product was detected by SDS-PAGE analysis and immunofluoresence.This experiment was expected to provide a new targeted drug for the clinical treatment of breast cancer.

关 键 词：SCFV PE40 乳腺癌 免疫毒素 

分 类 号：R730.3[医药卫生—肿瘤]