淫藤骨痹康方有效部位群在正常大鼠与急性血瘀大鼠体内药物化学对比研究  

Comparison of Pharmaceutical Chemistry Difference of Effective Parts from Yin Teng Gu Bi Kang Prescription in Normal Rats and Rats with Acute Blood Stasis

作　　者：王元清[1,2] 严建业[1] 李顺祥[1] 罗堃[1] 彭买姣[1] 徐菲[1] 

机构地区：[1]湖南中医药大学药学院中药现代化重点实验室,湖南长沙410208 [2]中南林业科技大学生命科学与技术学院生物技术与工程实验室,湖南长沙410004

出　　处：《中药材》2013年第12期1973-1978,共6页Journal of Chinese Medicinal Materials

基　　金：湖南省科技厅项目(2009TP4051-1);长沙市科技计划项目(k1003034-31);湖南省高校"中药新药创制与资源综合持续利用"科技创新团队资助(湘教通[2010]212号);国家中医药管理局"药用植物学";"中药药剂学"重点学科资助(国中医药发[2009]30号);湖南省"中药学"重点学科资助(湘教通[2011]76号)

摘　　要：目的:研究淫藤骨痹康方有效部位群在正常大鼠与急性血瘀大鼠体内药物化学差异性,阐明其活血作用的药效物质基础。方法:采用HPLC法建立淫藤骨痹康方有效部位群在正常生理状态与急性血瘀病理状态下的药物化学特征指纹图谱,并对体内外色谱峰进行对比分析。结果:在正常生理状态下,5个批次的含药血浆样品中有14个共有色谱峰,其中3个峰来自于空白血浆,9个峰为淫藤骨痹康方的原型成分,2个峰为其代谢产物峰或产生的新成分峰,经对照品对照,12 min处的峰为阿魏酸;在急性血瘀病理状态下,5个批次的含药血浆样品中有14个共有色谱峰,其中3个峰来自于空白血浆,9个峰为淫藤骨痹康方的原型成分,2个峰为其代谢产物峰或产生的新成分峰,经对照品对照,12 min处的峰为阿魏酸,32 min处的峰为淫羊藿苷;病理和正常生理状态下扣除空白血浆峰后的共有峰有10个,大多数共有峰峰面积病理状态下明显强于正常生理状态下,其中有3个共有峰峰面积具有显著性差异(P<0.05)。结论:血瘀影响淫藤骨痹康方有效部位群中大多数成分的吸收与代谢;其入血原型成分与代谢新成分可能为其活血作用的药效物质。Objective： To elucidate the material basis of Yin Teng Gu Bi Kang Prescription（ YTGBKP） for efficacy of promoting blood circulation by means of comparing the pharmaceutical chemistry difference of effective parts in normal rats and rats with acute blood stasis. Methods： The pharmaceutical chemistry fingerprints of effective parts under physiological and pathological status（ acute blood stasis） were established by HPLC,and the in vitro and in vivo chromatographic peaks were compared and analyzed. Results： Five batches of drug-containing plasma samples had 14 chromatographic peaks under normal physiological status,among which 3 rooted in plasma,9 existed originally in YTGBKP,2 were metabolites. The compound with retention time at 12 min was identified as ferulic acid by comparing with reference standard; While under pathological status（ acute blood stasis）,five batches of the drug-containing plasma samples had 14 chromatographic peaks,among which 3 rooted in plasma,9 existed originally in YTGBKP,2 were metabolites. The compounds with retention time at 12 min and 32 min were identified as ferulic acid and icariin respectively by comparing with reference standards. There were 10 common peaks under normal physiological and pathological status（ acute blood stasis） excluding peaks in blank plasma. The intensity of the common peaks produced under pathological status was stronger than that under normal physiological status significantly; Variance analysis showed that there were significant differences（ P 0. 05） in peak areas of 3 peaks. Conclusion： Blood stasis has influence on the absorption and metabolism of most ingredients from YTGBKP; Prototypes and metabolites may be the effective substance on promoting blood circulation.

关 键 词：淫藤骨痹康方 有效部位群 正常生理 急性血瘀 药物化学 指纹图谱 

分 类 号：R285.5[医药卫生—中药学]