槲皮素联合白藜芦醇逆转白血病细胞株HL-60-ADM耐药性的影响  

Effects of Quercetin and Resveratrol combined Reversing Drug Resistance of Leukemia Cell Line HL-60-ADM

作　　者：张复华[1] 尹松梅[2] 牛国敏[1] 杨国雷[1] 凌奕文[1] 李蕊[1] 徐嘉愉 

机构地区：[1]南方医科大学附属南海医院,广东佛山528200 [2]中山大学附属孙逸仙纪念医院,广东广州510120

出　　处：《中国医药指南》2014年第11期38-39,共2页Guide of China Medicine

摘　　要：目的：研究槲皮素（Quercetin，Que）及白藜芦醇（Resveratrol，Res）单用及联用对HL-60-A细胞株耐药性的影响。方法不同浓度阿霉素（adriamycin，ADM）孵育HL-60和HL-60-A细胞48 h后CCK-8法测定细胞存活率；Que、Res单用或联用，及加用ADM 48 h测定HL-60-A细胞存活率。结果 ADM作用48 h后，HL-60-A较HL-60细胞IC50明显增高增加提示为耐药细胞株；Que及Res在12.5～150μmol/L时呈剂量依赖性杀伤HL-60-A细胞，Que毒性作用更强，二者可以进一步增强杀伤效应；Que及Res低剂量时可增强ADM对HL-60-A敏感性，二者联用进一步增强ADM的杀伤效应。结论 Que及Res均可逆转白血病细胞耐药性，Que作用较强，二者联合可更有效逆转肿瘤耐药。Objective The recent study showed that querctin and resveratrol can reverse multidug resistance in leukemia. The aim of this study was to investigate the reversal multidug resistance effect by querctin and resveratrol. Methods CCK-8 assay was used to compare the toxic effect of querstin or resveratrol alone with that in combination with adriamycin on HL-60-A cells. Results Compared to HL-60 cell line, the IC50 value of adriamycin for HL-60-A increased signiifcantly, it showed that HL-60-A was multidug resistance cell line;querctin or resveratrol could inhibite cell proliferation of HL-60-A in a dose-dependent pattern, while the toxic effect of querctin combined with resveratrol was greater than that of the cells treated with querstin or resveratrol alone;the low-dose of querctin and resveratrol could enhance the sensitivity of adriamycin on HL-60-A, and querstin combined with resveratrol could further enhance the toxic effect of adriamycin. Conclusion Querctin and resveratrol both illustrated signiifcant reversal effect on the leukemia cell line, while the reversal effect of querctin was greater than resveratrol, the synergistic reversal effect of querstin combined with resveratrol was determined.

关 键 词：槲皮素 白藜芦醇 急性白血病 多药耐药 

分 类 号：R733.71[医药卫生—肿瘤]