五种DNA提取方法对鱼加工制品DNA提取效果的比较  

Evaluation of Five DNA Extraction Methods for Different Processed Fish Samples

作　　者：李进波[1,2] 盛婧 李想[2] 潘良文[2] 吕蓉[2] 杨捷琳[2] 

机构地区：[1]华东理工大学生物工程学院,上海200237 [2]上海出入境检验检疫局,上海200135 [3]上海市亭新中学,上海201505

出　　处：《生物技术通报》2014年第4期43-49,共7页Biotechnology Bulletin

基　　金：国家"十二五"农村领域国家科技计划课题(2011AA100807);上海市技术性贸易措施应对专项(12TBT011)

摘　　要：比较5种DNA提取方法对各种鱼加工制品的DNA提取效果,为后续检测提供更加有效的DNA提取方案。以11种不同加工方式和加工程度的鱼制品为样本,采用5种DNA提取方法——CTAB法以及4种常用市售试剂盒进行DNA提取。对DNA的提取质量、浓度和用于PCR扩增的效果及方法的可操作性等方面进行比较研究。结果显示,5种方法均可用于大多数鱼加工制品的DNA提取,但每种方法各有利弊。因此认为没有一种DNA提取试剂盒可以广泛地对所有类型鱼制品样品均有非常好的提取效果。It was to find more effective method for DNA extraction, five methodologies for DNA extraction from eleven different processed fish samples were compared. Five methods, including CTAB method and four commercial DNA extraction kits were employed and compared on the extraction of DNA from 11 processed fish samples, such as boiled, oil immersed, fumed, roasted, dried and salt-dried fish etc. The quality and quantity of the extracted DNA were evaluated by measurement of the absorbance at 230, 260 and 280 nm, and by qualitative PCR. The result showed that five methods can all be used to extract from the most processed fish samples, but the quality and quantity of the extracted DNA were different. So every method has its benefits and disadvantages.

关 键 词：鱼加工制品 DNA提取 DNA纯化 PCR 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]